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Proceedings 2017, 1(4), 530; doi:10.3390/proceedings1040530

Characterization of Retinal Pigmented Epithelium Cells Density on a MicroElectrode Array Using Impedance Spectroscopy

1
ESIEE—ESYCOM-Université Paris Est, Noisy-le-Grand, France
2
Institut de la Vision, Paris, France
Presented at the Eurosensors 2017 Conference, Paris, France, 3–6 September 2017.
*
Author to whom correspondence should be addressed.
Published: 28 August 2017
Download PDF [1807 KB, uploaded 28 August 2017]

Abstract

This work is aiming at using impedance measurements on MicroElectrode Array (MEA) in order to evaluate the cellular density at its surface. As the context of these measurements is retinal implants, the retinal pigmented epithelium cells (RPE) are used as cellular model. We measured the impedance changes at 4 different locations of the MEA during 13 days (approximately one measure every two days) and the cell density by manually counting them by microscope imaging taken just after the impedance measurement. A correlation between the cell density and the impedance measurement’s change can be observed. The electrode interfaces and the biological cells are associated with an electrical model taking into account the ionic conduction (resistance) and polarization effects (capacitance).
Keywords: impedance spectroscopy; MicroElectrode Array; retinal pigment epithelium cells impedance spectroscopy; MicroElectrode Array; retinal pigment epithelium cells
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Boutzen, J.; Valet, M.; Fradot, V.; Rousseau, L.; Français, O.; Picaud, S.; Lissorgues, G. Characterization of Retinal Pigmented Epithelium Cells Density on a MicroElectrode Array Using Impedance Spectroscopy. Proceedings 2017, 1, 530.

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