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Proceedings 2017, 1(4), 509; doi:10.3390/proceedings1040509

Real-Time QCM Measurements of Rolling Circle Amplification Products

1
Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, SE-171 65 Solna, Sweden
2
QCM Laboratory, Järfälla, Sweden
Presented at the Eurosensors 2017 Conference, Paris, France, 3–6 September 2017.
*
Author to whom correspondence should be addressed.
Published: 9 August 2017
(This article belongs to the Proceedings of Eurosensors 2017)
Download PDF [676 KB, uploaded 6 September 2017]

Abstract

We demonstrate real-time experimental evidence for mass underestimation, to analyse high molecular weight biomolecules while using Quartz Crystal Microbalance (QCM) platform. For this, we present an experimental evidence with an assay consisting of an aptamer-antibody sandwich model with norovirus-like particles (NoVLPs). We combine this with the proximity ligation assay to generate bulky rolling circle products on a gold spot centralized quartz resonator surface. Real-time monitoring of the assay build-up reflects that bulky NoVLPs and amplified oligonucleotides generate fluctuating signals during measurement using a microfluidic QCM sensor. Such an understanding of mass underestimation for heavier molecules becomes indispensable to get deeper insights into molecular interactions in biotechnology studied using QCM devices.
Keywords: quartz crystal microbalance; mass underestimation; rolling circle amplification; proximity ligation assay; norovirus-like particles quartz crystal microbalance; mass underestimation; rolling circle amplification; proximity ligation assay; norovirus-like particles
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Madaboosi, N.; Neumann, F.; Hernández-Neuta, I.; Salas, J.; Mecea, V.; Nilsson, M. Real-Time QCM Measurements of Rolling Circle Amplification Products. Proceedings 2017, 1, 509.

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