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Proceedings 2017, 1(4), 499; doi:10.3390/proceedings1040499

Pericellular Oxygen Monitoring during Low-Level Light Therapy in Cell Culture Using a Microsensor System

1
University of Freiburg, Freiburg, Germany
2
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria
Presented at the Eurosensors 2017 Conference, Paris, France, 3–6 September 2017.
*
Author to whom correspondence should be addressed.
Published: 21 August 2017
Download PDF [1590 KB, uploaded 29 August 2017]

Abstract

An electrochemical microsensor system to monitor the pericellular oxygen concentration of fibroblasts during low-level light therapy in vitro was developed. The system provides in-sight into the metabolism of the cells during and in consequence of illumination with visible red light. This approach is a unique method for real-time investigations of cellular respiration during light therapy. The presented sensor system features direct amperometric measurements by using chronoamperometric protocols for long-term stability. The oxygen measurements do not show a disturbance by light.
Keywords: pericellular oxygen measurement; electrochemical; low-level light therapy; cell culture pericellular oxygen measurement; electrochemical; low-level light therapy; cell culture
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Marzioch, J.; Kramer, F.; Dungel, P.; Kieninger, J.; Urban, G.A. Pericellular Oxygen Monitoring during Low-Level Light Therapy in Cell Culture Using a Microsensor System. Proceedings 2017, 1, 499.

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