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Proceedings 2017, 1(10), 1036; doi:10.3390/proceedings1101036

Development and Characterization of Paclitaxel-Loaded PLGA Nanoparticles and Cytotoxicity Assessment By MTT Assay On A549 Cell Line

1
Department of Pharmaceutical Technology, Faculty of Pharmacy, Erciyes University, Kayseri 38030, Turkey
2
Department of Pharmacognosy, Faculty of Pharmacy, Erciyes University, Kayseri 38030, Turkey
3
Department of Pharmaceutical Technology, Faculty of Pharmacy, Hacettepe University, Ankara 06100, Turkey
Presented at the 2nd International Conference on Natural Products for Cancer Prevention and Therapy, Kayseri, Turkey, 8–11 November 2017.
*
Author to whom correspondence should be addressed.
Published: 10 November 2017
Download PDF [162 KB, uploaded 13 November 2017]

Abstract

Abstract: Paclitaxel (PTX) is a cytotoxic agent derived from Taxus brevifolia used for lung cancer. Poly d,l-lactide- co-glycolide (PLGA) is an FDA-approved biodegradable polymer used for preparation of nanoparticles carrying cytotoxic drug agents. The purpose of this study was to evaluate the effect of PTX solution and paclitaxel-loaded PLGA nanoparticles (PTX-PLGA-NPs) on A549 cells. PTX-PLGA-NPs were prepared using nanoprecipation technique as described by Fessi et al. Particle size, polydispersity index, zeta potential and encapsulation efficiency of nanoparticles were measured. Cytotoxic effects of the various concentrations of PTX and PTX-PLGA-NPs on A549 cell line were determined by MTT assay. The cells were treated with various PTX and PTX-PLGA-NPs concentrations (1–10 µg/mL) for 24 h. Particle size, polydispersity index, zeta potential values and encapsulation efficiency were found as 267 ± 14 nm, 0.29, −28 ± 3 mV and 76% respectively. According to MTT results, the cytotoxicity effect of PTX-PLGA-NPs on A549 cells significantly increased in comparison to PTX solution at all concentrations. The enhancement of cytotoxicity can be explained by the sustained release of the PTX from the PLGA nanoparticles and the increase in PTX amount inside the A549 cells. It can be concluded that PTX-PLGA-NPs can be promising systems for anticancer therapy.
Keywords: PLGA; nanoparticle; A549; MTT; cytotoxicity PLGA; nanoparticle; A549; MTT; cytotoxicity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Ünal, S.; Yücel, Ç.; Tekeli, M.Ç.; Karatoprak, G.Ş.; Aktaş, Y.; Bilensoy, E. Development and Characterization of Paclitaxel-Loaded PLGA Nanoparticles and Cytotoxicity Assessment By MTT Assay On A549 Cell Line. Proceedings 2017, 1, 1036.

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