Next Article in Journal / Special Issue
Molecular and Microscopic Insights into the Formation of Soil Organic Matter in a Red Pine Rhizosphere
Previous Article in Journal / Special Issue
Legacy of Rice Roots as Encoded in Distinctive Microsites of Oxides, Silicates, and Organic Matter
Article Menu

Export Article

Open AccessArticle
Soils 2017, 1(1), 3; doi:10.3390/soils1010003

Evidence for the Root-Uptake of Arsenite at Lateral Root Junctions and Root Apices in Rice (Oryza sativa L.)

1
Department of Plant & Soil Sciences, University of Delaware, Newark, DE 19716, USA
2
Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, USA
3
Stanford Synchrotron Radiation Lightsource, Menlo Park, CA 94025, USA
*
Author to whom correspondence should be addressed.
Received: 12 July 2017 / Revised: 8 August 2017 / Accepted: 11 August 2017 / Published: 14 August 2017
(This article belongs to the Special Issue Rhizosphere Processes)
View Full-Text   |   Download PDF [2898 KB, uploaded 14 August 2017]   |  

Abstract

The uptake of arsenite (As(III)i) at the Casparian band via Lsi1 and Lsi2 Si transporters is responsible for ~75% of shoot As(III)i uptake in rice and, therefore, ~25% of shoot As(III)i is taken up by other transport pathways. We hypothesized that areas devoid of Casparian bands—lateral root junctions and root apices—can transport As(III)i into roots. We analyzed the elemental distribution and As concentration, speciation, and localization in rice roots from soil-grown and solution-grown plants. With solution-grown plants dosed with As(III)i, we sectioned roots as a function of distance from the root apex and analyzed the cross-sections using confocal microscopy coupled to synchrotron X-ray fluorescence imaging and spectroscopy. We observed elevated As(III)i associated with lateral root junctions and root apices in rice. As(III)i entered the stele at lateral root junctions and radially permeated the root interior in cross-sections 130–140 µm from the root apex that are devoid of Casparian bands. Our findings suggest that lateral root junctions and rice root apices are hot-spots for As(III)i transport into rice roots, but the contribution to shoot As requires further research. View Full-Text
Keywords: rice; arsenite; transport; synchrotron XRF imaging rice; arsenite; transport; synchrotron XRF imaging
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Seyfferth, A.L.; Ross, J.; Webb, S.M. Evidence for the Root-Uptake of Arsenite at Lateral Root Junctions and Root Apices in Rice (Oryza sativa L.). Soils 2017, 1, 3.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Soils EISSN 2411-5126 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top