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Head Kidney Transcriptome Analysis and Characterization for the Sub-Antarctic Notothenioid Fish Eleginops maclovinus

1
Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia 5090000, Chile
2
Escuela de Graduados, Programa de Doctorado en Ciencias de la Acuicultura, Universidad Austral de Chile, Av. Los Pinos s/n Balneario Pelluco, Puerto Montt 5480000, Chile
3
Centro Fondap de Investigación de Altas Latitudes (IDEAL), Universidad Austral de Chile, Valdivia 5090000, Chile
4
Instituto de Farmacología y Morfofisiología, Universidad Austral de Chile, Valdivia 5090000, Chile
5
Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Valdivia 5090000, Chile
6
Interdisciplinary Center for Aquaculture Research (INCAR), Concepción 4030000, Chile
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 2 January 2018 / Revised: 16 January 2018 / Accepted: 16 January 2018 / Published: 25 January 2018
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Abstract

This study describes de novo transcriptome sequencing and annotation analyses for the head kidney of the sub-Antarctic notothenioid fish Eleginops maclovinus, a sister group of the Antarctic notothenioid fish clade. Moreover, E. maclovinus is one of the most eurythermal and euryhaline representatives of the Notothenioidei suborder. RNA-seq data were generated by the 454 GS Junior system, resulting in 11,207 contigs that were then assembled by the Genomic Workbench CLC software. The transcriptome was annotated by BLASTing each sequence against the universal, non-redundant NCBI database (National Center for Biotechnology Information) using the AUSTRAL-omics computer cluster. A significant number of transcripts related to innate and adaptive immunity were found in the sequences, which could be used as references in future immunological studies in E. maclovinus. View Full-Text
Keywords: Eleginops maclovinus; transcriptome; notothenioid; immunity; head kidney Eleginops maclovinus; transcriptome; notothenioid; immunity; head kidney
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Martínez, D.; Pontigo, J.P.; Morera, F.J.; Yañéz, A.; Vargas-Chacoff, L. Head Kidney Transcriptome Analysis and Characterization for the Sub-Antarctic Notothenioid Fish Eleginops maclovinus. Fishes 2018, 3, 8.

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