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Magnetochemistry 2018, 4(1), 2; doi:10.3390/magnetochemistry4010002

Accelerating NMR-Based Structural Studies of Proteins by Combining Amino Acid Selective Unlabeling and Fast NMR Methods

1
NMR Research Centre, Indian Institute of Science, Bangalore 560012, India
2
Solid State and Structural Chemistry Unit, Indian Institute of Science, Bangalore 560012, India
*
Authors to whom correspondence should be addressed.
Received: 30 October 2017 / Revised: 4 December 2017 / Accepted: 7 December 2017 / Published: 26 December 2017
(This article belongs to the Special Issue Nuclear Magnetic Resonance Spectroscopy)
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Abstract

In recent years, there has been a growing interest in fast acquisition and analysis of nuclear magnetic resonance (NMR) spectroscopy data for high throughput protein structure determination. Towards this end, rapid data collection techniques and methods to simplify the NMR spectrum such as amino acid selective unlabeling have been proposed recently. Combining these two approaches can speed up further the structure determination process. Based on this idea, we present three new two-dimensional (2D) NMR experiments, which together provide 15N, 1HN, 13Cα, 13Cβ, 13C′ chemical shifts for amino acid residues which are immediate C-terminal neighbors (i + 1) of residues that are selectively unlabeled. These experiments have high sensitivity and can be acquired rapidly using the methodology of G-matrix Fourier transform (GFT) NMR spectroscopy combined with non-uniform sampling (NUS). This is a first study involving the application of fast NMR methods to proteins samples prepared using a specific labeling scheme. Taken together, this opens up new avenues to using the method of selective unlabeling for rapid resonance assignment of proteins. View Full-Text
Keywords: selective unlabeling; GFT NMR; non-uniform sampling; fast NMR methods; protein resonance assignments selective unlabeling; GFT NMR; non-uniform sampling; fast NMR methods; protein resonance assignments
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Krishnarjuna, B.; Chandra, K.; Atreya, H.S. Accelerating NMR-Based Structural Studies of Proteins by Combining Amino Acid Selective Unlabeling and Fast NMR Methods. Magnetochemistry 2018, 4, 2.

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