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Fermentation 2018, 4(3), 58; https://doi.org/10.3390/fermentation4030058

Impact of Organic Acids Supplementation to Hardwood Spent Sulfite Liquor as Substrate for the Selection of Polyhydroxyalkanoates-Producing Organisms

1
Departamento de Química/CICECO, Universidade de Aveiro, Campus Universitário de Santiago, P-3810-193 Aveiro, Portugal
2
Laboratório Associado para a Química Verde, Rede de Química e Tecnologia (LAQV, REQUIMTE), Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, P-2829-516 Caparica, Portugal
3
Water Research Institute-Consiglio Nazionale della Ricerche (CNR), Via Salaria Km 29,300, 00015 Monterotondo, Italy
*
Author to whom correspondence should be addressed.
Received: 12 July 2018 / Revised: 18 July 2018 / Accepted: 18 July 2018 / Published: 24 July 2018
(This article belongs to the Special Issue Microbial Production of Added-value Products from Renewable Resources)
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Abstract

The effectiveness of polyhydroxyalkanoates (PHAs) production process from a waste stream is determined by the selection of a suitable mixed microbial culture (MMC). In this work, a feedstock from the paper industry, hardwood spent sulfite liquor (HSSL), supplemented with short-chain organic acids (SCOAs) to simulate a fermented effluent, was used as substrate to enrich a MMC in PHA-storing microorganisms. A stable culture was quickly established, and during the accumulation step the selected MMC reached a maximum PHA content of 34.6% (3HB:3HV-76:24). The bacterial community was analyzed through FISH analysis. Bacteria belonging to the four main classes were identified: Betaproteobacteria (44.7 ± 2.7%), Alphaproteobacteria (13.6 ± 1.3%) and Gammaproteobacteria (2.40 ± 1.1%) and Bacteroidetes (9.20 ± 3.8%). Inside the Betaproteobacteria class, Acidovorax (71%) was the dominant genus. View Full-Text
Keywords: polyhydroxyalkanoates; mixed microbial cultures; hardwood spent sulfite liquor; short-chain organic acids; Fluorescence in situ Hybridization (FISH) polyhydroxyalkanoates; mixed microbial cultures; hardwood spent sulfite liquor; short-chain organic acids; Fluorescence in situ Hybridization (FISH)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Queirós, D.; Rangel, C.; Costa Lemos, P.; Rossetti, S.; Seuanes Serafim, L. Impact of Organic Acids Supplementation to Hardwood Spent Sulfite Liquor as Substrate for the Selection of Polyhydroxyalkanoates-Producing Organisms. Fermentation 2018, 4, 58.

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