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Fermentation 2018, 4(2), 45; https://doi.org/10.3390/fermentation4020045

Enhancement of the Efficiency of Bioethanol Production by Saccharomyces cerevisiae via Gradually Batch-Wise and Fed-Batch Increasing the Glucose Concentration

1
Department of Food Science, Yuanpei University of Medical Technology, Hsinchu 300, Taiwan
2
Institute of Food Science, Yuanpei University of Medical Technology, Hsinchu 300, Taiwan
3
Department of Food Science, Tunghai University, Taichung 407, Taiwan
4
Department of Nursing, Mackay Junior College of Medicine, Nursing, and Management, Taipei 11260, Taiwan
*
Author to whom correspondence should be addressed.
Received: 24 May 2018 / Revised: 8 June 2018 / Accepted: 11 June 2018 / Published: 13 June 2018
(This article belongs to the Special Issue Bioprocess and Fermentation Monitoring)
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Abstract

High initial glucose concentrations may inhibit glucose utilization and decrease ethanol fermentation efficiency. To minimize substrate inhibition, the effects of feeding yeast with different glucose concentrations on the ethanol production by batch and fed-batch cultures in a 5-L fermentor were investigated. When a batch culture system with Saccharomyces cerevisiae was used for ethanol fermentation with glucose concentrations ranging 10–260 g/L, as a result, 0.2–7.0 g/L biomass and 5.1–115.0 g/L ethanol were obtained. However, substrate inhibition was observed with the initial glucose concentrations greater than 200 g/L in the fermentative media. When a fed-batch culture system (an initial glucose concentration of 180 g/L and total glucose concentration of 260 g/L) was performed, the maximum ethanol concentrations and ethanol yield were significantly higher than those of the batch cultures. The cell biomass, maximum ethanol concentration, and ethanol yields for the fed-batch fermentation cultures were 8.3 g/L, 130.1 g/L and 51% (100% of the theoretical value), respectively. The results indicated that high ethanol concentration and ethanol yield could be achieved by the fed-batch cultures with total glucose concentrations up to 260 g/L. View Full-Text
Keywords: bioethanol; glucose inhibition; batch culture; fed-batch culture; fermentor bioethanol; glucose inhibition; batch culture; fed-batch culture; fermentor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Chang, Y.-H.; Chang, K.-S.; Chen, C.-Y.; Hsu, C.-L.; Chang, T.-C.; Jang, H.-D. Enhancement of the Efficiency of Bioethanol Production by Saccharomyces cerevisiae via Gradually Batch-Wise and Fed-Batch Increasing the Glucose Concentration. Fermentation 2018, 4, 45.

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