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Fermentation 2018, 4(2), 41; https://doi.org/10.3390/fermentation4020041

Fluorinated Phenylalanine Precursor Resistance in Yeast

Biomolecular Sciences Institute, Florida International University, Miami 33199, FL, USA
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Received: 27 April 2018 / Revised: 2 June 2018 / Accepted: 4 June 2018 / Published: 9 June 2018
(This article belongs to the Special Issue Yeast Biotechnology 2.0)
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Abstract

Development of a counter-selection method for phenylalanine auxotrophy could be a useful tool in the repertoire of yeast genetics. Fluorinated and sulfurated precursors of phenylalanine were tested for toxicity in Saccharomyces cerevisiae. One such precursor, 4-fluorophenylpyruvate (FPP), was found to be toxic to several strains from the Saccharomyces and Candida genera. Toxicity was partially dependent on ARO8 and ARO9, and correlated with a strain’s ability to convert FPP into 4-fluorophenylalanine (FPA). Thus, strains with deletions in ARO8 and ARO9, having a mild phenylalanine auxotrophy, could be separated from a culture of wild-type strains using FPP. Tetrad analysis suggests FPP resistance in one strain is due to two genes. Strains resistant to FPA have previously been shown to exhibit increased phenylethanol production. However, FPP resistant isolates did not follow this trend. These results suggest that FPP could effectively be used for counter-selection but not for enhanced phenylethanol production. View Full-Text
Keywords: fluorinated compounds; counter selection; phenylalanine; phenylethanol; yeast fluorinated compounds; counter selection; phenylalanine; phenylethanol; yeast
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Murdoch, I.S.; Powers, S.L.; Welch, A.Z. Fluorinated Phenylalanine Precursor Resistance in Yeast. Fermentation 2018, 4, 41.

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