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Fermentation 2017, 3(2), 25; doi:10.3390/fermentation3020025

Amylase Production from Thermophilic Bacillus sp. BCC 021-50 Isolated from a Marine Environment

1
College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China
2
Institute of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro 76080, Sindh, Pakistan
3
Department of Chemical Engineering, University of Engineering & Technology, KSK Campus, Lahore 54890, Pakistan
4
Department of Botany, Shah Abdul Latif University, Khairpur Mir’s 66111, Sindh, Pakistan
*
Author to whom correspondence should be addressed.
Academic Editors: Clemencia Chaves-López and Annalisa Serio
Received: 27 March 2017 / Revised: 18 May 2017 / Accepted: 26 May 2017 / Published: 1 June 2017
(This article belongs to the Special Issue Fermentation and Bioactive Metabolites)
View Full-Text   |   Download PDF [1767 KB, uploaded 1 June 2017]   |  

Abstract

The high cost of fermentation media is one of the technical barriers in amylase production from microbial sources. Amylase is used in several industrial processes or industries, for example, in the food industry, the saccharification of starchy materials, and in the detergent and textile industry. In this study, marine microorganisms were isolated to identify unique amylase-producing microbes in starch agar medium. More than 50 bacterial strains with positive amylase activity, isolated from marine water and soil, were screened for amylase production in starch agar medium. Bacillus sp. BCC 021-50 was found to be the best amylase-producing strain in starch agar medium and under submerged fermentation conditions. Next, fermentation conditions were optimized for bacterial growth and enzyme production. The highest amylase concentration of 5211 U/mL was obtained after 36 h of incubation at 50 °C, pH 8.0, using 20 g/L molasses as an energy source and 10 g/L peptone as a nitrogen source. From an application perspective, crude amylase was characterized in terms of temperature and pH. Maximum amylase activity was noted at 70 °C and pH 7.50. However, our results show clear advantages for enzyme stability in alkaline pH, high-temperature, and stability in the presence of surfactant, oxidizing, and bleaching agents. This research contributes towards the development of an economical amylase production process using agro-industrial residues. View Full-Text
Keywords: Bacillus sp. BCC 021-50; amylase production; characterization and marine organisms Bacillus sp. BCC 021-50; amylase production; characterization and marine organisms
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Simair, A.A.; Khushk, I.; Qureshi, A.S.; Bhutto, M.A.; Chaudhry, H.A.; Ansari, K.A.; Lu, C. Amylase Production from Thermophilic Bacillus sp. BCC 021-50 Isolated from a Marine Environment. Fermentation 2017, 3, 25.

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