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Non-Coding RNA 2018, 4(2), 9; https://doi.org/10.3390/ncrna4020009

Strengths and Weaknesses of the Current Strategies to Map and Characterize R-Loops

UMR5239 CNRS, Université de Lyon, ENS-Lyon, 46 allée d’Italie, 69007 Lyon, France
Received: 1 March 2018 / Revised: 22 March 2018 / Accepted: 23 March 2018 / Published: 27 March 2018
(This article belongs to the Special Issue Genomic instability and Non-Coding RNA)
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Abstract

R-loops are evolutionarily conserved three-stranded structures that result from the formation of stable DNA:RNA hybrids in the genome. R-loops have attracted increasing interest in recent years as potent regulators of gene expression and genome stability. In particular, their strong association with severe replication stress makes them potential oncogenic structures. Despite their importance, the rules that govern their formation and their dynamics are still controversial and an in-depth description of their direct impact on chromatin organization and DNA transactions is still lacking. To better understand the diversity of R-loop functions, reliable, accurate, and quantitative mapping techniques, as well as functional assays are required. Here, I review the different approaches that are currently used to do so and to highlight their individual strengths and weaknesses. In particular, I review the advantages and disadvantages of using the S9.6 antibody to map R-loops in vivo in an attempt to propose guidelines for best practices. View Full-Text
Keywords: R-loops; S9.6; RNase H1; DRIP; R-ChIP R-loops; S9.6; RNase H1; DRIP; R-ChIP
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Vanoosthuyse, V. Strengths and Weaknesses of the Current Strategies to Map and Characterize R-Loops. Non-Coding RNA 2018, 4, 9.

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