Next Article in Journal
MicroRNA and Breast Cancer: Understanding Pathogenesis, Improving Management
Previous Article in Journal
Welcome to the New Journal Non-Coding RNA!
Article Menu

Export Article

Open AccessArticle
Non-Coding RNA 2015, 1(1), 4-16; doi:10.3390/ncrna1010004

Repression of RNA Polymerase II Transcription by B2 RNA Depends on a Specific Pattern of Structural Regions in the RNA

University of Colorado, Department of Chemistry and Biochemistry, 596 UCB, Boulder, CO 80309, USA
*
Authors to whom correspondence should be addressed.
Academic Editor: George A. Calin
Received: 15 December 2014 / Revised: 7 January 2015 / Accepted: 19 January 2015 / Published: 28 January 2015
View Full-Text   |   Download PDF [462 KB, uploaded 28 January 2015]   |  

Abstract

B2 RNA is a mouse non-coding RNA that binds directly to RNA polymerase II (Pol II) and represses transcription by disrupting critical interactions between the polymerase and promoter DNA. How the structural regions within B2 RNA work together to mediate transcriptional repression is not well understood. To address this question, we systematically deleted structural regions from B2 RNA and determined the effects on transcriptional repression using a highly purified Pol II in vitro transcription system. Deletions that compromised the ability of B2 RNA to function as a transcriptional repressor were also tested for their ability to bind directly to Pol II, which enabled us to distinguish regions uniquely important for repression from those important for binding. We found that transcriptional repression requires a pattern of RNA structural motifs consisting of an extended single-stranded region bordered by two stem‑loops. Hence, there is modularity in the function of the stem-loops in B2 RNA—when one stem‑loop is deleted, another can take its place to enable transcriptional repression. View Full-Text
Keywords: non-coding RNA; transcription; RNA polymerase II; B2 RNA; repressor; SINE non-coding RNA; transcription; RNA polymerase II; B2 RNA; repressor; SINE
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Ponicsan, S.L.; Kugel, J.F.; Goodrich, J.A. Repression of RNA Polymerase II Transcription by B2 RNA Depends on a Specific Pattern of Structural Regions in the RNA. Non-Coding RNA 2015, 1, 4-16.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Non-Coding RNA EISSN 2311-553X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top