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J. Fungi 2018, 4(3), 108; https://doi.org/10.3390/jof4030108

Standardization of G. mellonella Larvae to Provide Reliable and Reproducible Results in the Study of Fungal Pathogens

1
Biosystems Technology Ltd., 1 Colleton Crescent, Exeter EX2 4DG, UK
2
College of Life and Environmental Science, University of Exeter, Stocker Road, Exeter EX4 4QD, UK
*
Author to whom correspondence should be addressed.
Received: 13 August 2018 / Revised: 31 August 2018 / Accepted: 5 September 2018 / Published: 6 September 2018
(This article belongs to the Special Issue Alternative Models for the Study of Fungal Host-Pathogen Interactions)
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Abstract

In the past decade, Galleria mellonella (wax moth) larvae have become widely used as a non-mammalian infection model. However, the full potential of this infection model has yet to be realised, limited by the variable quality of larvae used and the lack of standardised procedures. Here, we review larvae suitable for research, protocols for dosing larvae, and methods for scoring illness in larvae infected with fungal pathogens. The development of standardised protocols for carrying out our experimental work will allow high throughput screens to be developed, changing the way in which we evaluate panels of mutants and strains. It will also enable the in vivo screening of potential antimicrobials at an earlier stage in the research and development cycle. View Full-Text
Keywords: Galleria mellonella; infection model; fungi; genome; pathological score; end point Galleria mellonella; infection model; fungi; genome; pathological score; end point
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Champion, O.L.; Titball, R.W.; Bates, S. Standardization of G. mellonella Larvae to Provide Reliable and Reproducible Results in the Study of Fungal Pathogens. J. Fungi 2018, 4, 108.

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