Next Article in Journal
Pseudogymnoascus destructans: Causative Agent of White-Nose Syndrome in Bats Is Inhibited by Safe Volatile Organic Compounds
Previous Article in Journal
Mucormycosis: Battle with the Deadly Enemy over a Five-Year Period in India
Article Menu

Export Article

Open AccessArticle
J. Fungi 2018, 4(2), 47; doi:10.3390/jof4020047

Microenvironment Responsive Modulations in the Fatty Acid Content, Cell Surface Hydrophobicity, and Adhesion of Candida albicans Cells

Research Laboratory 1, School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded431606, MS, India
*
Author to whom correspondence should be addressed.
Received: 2 February 2018 / Revised: 18 March 2018 / Accepted: 4 April 2018 / Published: 6 April 2018
View Full-Text   |   Download PDF [5409 KB, uploaded 12 April 2018]   |  

Abstract

Considering the significance in survival and virulence, we have made an attempt to understand modulations in the membrane and cell wall properties of Candida albicans hyphae induced by temperature (37 °C) and neutral pH and yeast form cells grown under low hydrostatic pressure (LHP). Our results suggest that cell surface hydrophobicity (CSH) and adhesion are dynamic properties determined largely by the microenvironment rather than morphological forms, citing the significance of variation in niche specific virulence. GC-MS analysis showed that 49 and 41 fatty acids modulated under hyphal form induced by temperature alone (37 °C) and neutral pH, respectively while that of 58 under yeast form cells under low hydrostatic pressure (LHP) (1800 Pa). Fatty acid and ergosterol data indicates that fluidity increases with increase in temperature (37 °C) and neutral pH i.e., saturated fatty acids and ergosterol decreases. Similarly, CSH and adhesion decrease in response to temperature (37 °C), pH 7, and LHP compared to controls, irrespective of morphological forms. In general, membranes were more rigid, and cell walls were more hydrophobic and adhesive in yeast form compared to hyphal form cells, except in case of yeast form cells grown under LHP. Yeast form cells grown under LHP are less hydrophobic and adhesive. View Full-Text
Keywords: cell surface hydrophobicity; adhesion; ergosterol; fatty acids; lipids; membrane fluidity cell surface hydrophobicity; adhesion; ergosterol; fatty acids; lipids; membrane fluidity
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Share & Cite This Article

MDPI and ACS Style

Shiradhone, A.B.; Ingle, S.S.; Zore, G.B. Microenvironment Responsive Modulations in the Fatty Acid Content, Cell Surface Hydrophobicity, and Adhesion of Candida albicans Cells. J. Fungi 2018, 4, 47.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
J. Fungi EISSN 2309-608X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top