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J. Fungi 2016, 2(1), 6; doi:10.3390/jof2010006

Proteomic Analysis of Pathogenic Fungi Reveals Highly Expressed Conserved Cell Wall Proteins

1
Department of Molecular Immunology, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA
2
Division of Infectious Diseases, City of Hope National Medical Center, Duarte, CA 91010, USA
3
California Institute for Medical Research, San Jose, CA 95128, USA
4
Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA 94305, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Luis V. Lopez-Llorca
Received: 24 November 2015 / Revised: 21 December 2015 / Accepted: 5 January 2016 / Published: 12 January 2016
(This article belongs to the Special Issue Fungal '-Omics': Is the Best Yet to Come?)
View Full-Text   |   Download PDF [4492 KB, uploaded 12 January 2016]   |  

Abstract

We are presenting a quantitative proteomics tally of the most commonly expressed conserved fungal proteins of the cytosol, the cell wall, and the secretome. It was our goal to identify fungi-typical proteins that do not share significant homology with human proteins. Such fungal proteins are of interest to the development of vaccines or drug targets. Protein samples were derived from 13 fungal species, cultured in rich or in minimal media; these included clinical isolates of Aspergillus, Candida, Mucor, Cryptococcus, and Coccidioides species. Proteomes were analyzed by quantitative MSE (Mass Spectrometry—Elevated Collision Energy). Several thousand proteins were identified and quantified in total across all fractions and culture conditions. The 42 most abundant proteins identified in fungal cell walls or supernatants shared no to very little homology with human proteins. In contrast, all but five of the 50 most abundant cytosolic proteins had human homologs with sequence identity averaging 59%. Proteomic comparisons of the secreted or surface localized fungal proteins highlighted conserved homologs of the Aspergillus fumigatus proteins 1,3-β-glucanosyltransferases (Bgt1, Gel1-4), Crf1, Ecm33, EglC, and others. The fact that Crf1 and Gel1 were previously shown to be promising vaccine candidates, underlines the value of the proteomics data presented here. View Full-Text
Keywords: proteomics; vaccine candidates; Aspergillus; Candida; fungal pathogens proteomics; vaccine candidates; Aspergillus; Candida; fungal pathogens
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Champer, J.; Ito, J.I.; Clemons, K.V.; Stevens, D.A.; Kalkum, M. Proteomic Analysis of Pathogenic Fungi Reveals Highly Expressed Conserved Cell Wall Proteins. J. Fungi 2016, 2, 6.

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