Next Article in Journal / Special Issue
LTD4 and TGF-β1 Induce the Expression of Metalloproteinase-1 in Chronic Rhinosinusitis via a Cysteinyl Leukotriene Receptor 1-Related Mechanism
Previous Article in Journal / Special Issue
Chronic Rhinosinusitis as a Crucial Symptom of Cystic Fibrosis—Case Report and Discussion on the Sinonasal Compartment as Site of Pseudomonas aeruginosa Acquisition into CF Airways
Article Menu

Export Article

Open AccessArticle
Sinusitis 2016, 1(1), 55-64; doi:10.3390/sinusitis1010055

Fatty Acid Composition of Cultured Fibroblasts Derived from Healthy Nasal Mucosa and Nasal Polyps

1
Institut d’Investigacions Biomèdiques August Pi y Sunyer (IDIBAPS),08036 Barcelona, Spain
2
Hospital Clinic, Universitat de Barcelona, 08036 Barcelona, Spain
3
Centro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (CIBERES), 08036 Barcelona, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Claudina A. Pérez Novo
Received: 26 November 2015 / Revised: 25 February 2016 / Accepted: 5 April 2016 / Published: 11 April 2016
(This article belongs to the Special Issue Chronic Rhinosinusitis: Clinical and Immunological Research)
View Full-Text   |   Download PDF [212 KB, uploaded 11 April 2016]

Abstract

Background: Fibroblasts from nasal polyps (NP) of asthma patients have reduced expression of cyclooxygenase 2 (COX-2) and production of prostaglandin E2 (PGE2). We hypothesized that the reported alterations are due to alterations in the availability of arachidonic acid (AA). Objective: The objective was to determine the fatty acid composition of airway fibroblasts from healthy subjects and from asthma patients with and without aspirin intolerance. Methods: We analyzed the fatty acid composition of cultured fibroblasts from non-asthmatics (n = 6) and from aspirin-tolerant (n = 6) and aspirin-intolerant asthmatics (n = 6) by gas chromatography-flame ionization detector. Fibroblasts were stimulated with acetyl salicylic acid (ASA). Results: The omega-6 fatty acids dihomo-gamma-linolenic acid (C20:3) and AA (C20:4), and omega-3 fatty acids docosapentaenoic acid (DPA) (C22:5) and docosahexaenoic acid (DHA) (C22:6) were significantly higher in NP fibroblasts than in fibroblasts derived from nasal mucosa. The percentage composition of the fatty acids palmitic acid (C16:0) and palmitoleic acid (C16:1) was significantly higher in fibroblasts from patients with NP and aspirin intolerance than in fibroblasts derived from the nasal NP of aspirin-tolerant patients. ASA did not cause changes in either omega-3 or omega-6 fatty acids. Conclusions. Our data do not support the hypothesis that a reduced production of AA in NP fibroblasts can account for the reported low production of PGE2 in nasal polyps. Whether the increased proportion of omega-3 fatty acids can contribute to reduced PGE2 production in nasal polyps by competitively inhibiting COX-2 and reducing the amount of AA available to the COX-2 enzyme remains to be elucidated. View Full-Text
Keywords: arachidonic acid; aspirin intolerance; asthma; cyclooxygenase; eicosanoids; fatty acids; fibroblast; inflammation; nasal mucosa; nasal polyps arachidonic acid; aspirin intolerance; asthma; cyclooxygenase; eicosanoids; fatty acids; fibroblast; inflammation; nasal mucosa; nasal polyps
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Jabr Ayyad, S.; Roca-Ferrer, J.; Picado, C. Fatty Acid Composition of Cultured Fibroblasts Derived from Healthy Nasal Mucosa and Nasal Polyps. Sinusitis 2016, 1, 55-64.

Show more citation formats Show less citations formats

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sinusitis EISSN 2309-107X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top