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Vet. Sci. 2016, 3(4), 34; doi:10.3390/vetsci3040034

Fluorescent Protein Expressing Rickettsia buchneri and Rickettsia peacockii for Tracking Symbiont-Tick Cell Interactions

Department of Entomology, University of Minnesota, 1980 Folwell Avenue, Saint Paul, MN 55108, USA
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Author to whom correspondence should be addressed.
Academic Editor: László Fodor
Received: 30 September 2016 / Revised: 10 November 2016 / Accepted: 14 November 2016 / Published: 17 November 2016
(This article belongs to the Special Issue Comparative Studies in Tick-Borne Diseases in Animals and Humans)
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Abstract

Rickettsiae of indeterminate pathogenicity are widely associated with ticks. The presence of these endosymbionts can confound a One Health approach to combatting tick-borne diseases. Genomic analyses of symbiotic rickettsiae have revealed that they harbor mutations in gene coding for proteins involved in rickettsial pathogenicity and motility. We have isolated and characterized two rickettsial symbionts—Rickettsia peacockii and R. buchneri—both from ticks using tick cell cultures. To better track these enigmatic rickettsiae in ticks and at the tick-mammal interface we transformed the rickettsiae to express fluorescent proteins using shuttle vectors based on rickettsial plasmids or a transposition system driving insertional mutagenesis. Fluorescent protein expressing R. buchneri and R. peacockii will enable us to elucidate their interactions with tick and mammalian cells, and track their location and movement within individual cells, vector ticks, and host animals. View Full-Text
Keywords: ticks; endosymbionts; Rickettsia buchneri; Rickettsia peacockii; transformation; shuttle vector; Himar 1 transposition; green fluorescent protein; mCherry fluorescent protein; spectinomycin; rifampin ticks; endosymbionts; Rickettsia buchneri; Rickettsia peacockii; transformation; shuttle vector; Himar 1 transposition; green fluorescent protein; mCherry fluorescent protein; spectinomycin; rifampin
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kurtti, T.J.; Burkhardt, N.Y.; Heu, C.C.; Munderloh, U.G. Fluorescent Protein Expressing Rickettsia buchneri and Rickettsia peacockii for Tracking Symbiont-Tick Cell Interactions. Vet. Sci. 2016, 3, 34.

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