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Bioengineering 2018, 5(1), 19; doi:10.3390/bioengineering5010019

Evaluation of Peripheral Blood and Cord Blood Platelet Lysates in Isolation and Expansion of Multipotent Mesenchymal Stromal Cells

1
Hellenic Cord Blood Bank, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27 Athens, Greece
2
Biotechnology division, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27 Athens, Greece
These authors contributed equally to this work as first authors.
*
Author to whom correspondence should be addressed.
Received: 7 November 2017 / Revised: 16 January 2018 / Accepted: 24 February 2018 / Published: 26 February 2018
(This article belongs to the Special Issue Mesenchymal Stem Cells in Tissue Regeneration)
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Abstract

Background: Multipotent Mesenchymal Stromal Cells (MSCs) are used in tissue engineering and regenerative medicine. The in vitro isolation and expansion of MSCs involve the use of foetal bovine serum (FBS). However, many concerns have been raised regarding the safety of this product. In this study, alternative additives derived either from peripheral or cord blood were tested as an FBS replacement. Methods: Platelet lysates (PL) from peripheral and cord blood were used for the expansion of MSCs. The levels of growth factors in peripheral blood (PB) and cord blood (CB) PLs were determined using the Multiple Reaction Monitoring (MRM). Finally, the cell doubling time (CDT), tri-lineage differentiation and phenotypic characterization of the MSCs expanded with FBS and PLs were determined. Results: MSCs treated with culture media containing FBS and PB-PL, were successfully isolated and expanded, whereas MSCs treated with CB-PL could not be maintained in culture. Furthermore, the MRM analysis yielded differences in growth factor levels between PB-PL and CB-PL. In addition, the MSCs were successfully expanded with FBS and PB-PL and exhibited tri-lineage differentiation and stable phenotypic characteristics. Conclusion: PB-PL could be used as an alternative additive for the production of MSCs culture medium applied to xenogeneic-free expansion and maintenance of MSCs in large scale clinical studies. View Full-Text
Keywords: Cord blood; Multiple Reaction Monitoring; multipotent Mesenchymal Stem Cells; peripheral blood; platelet lysate Cord blood; Multiple Reaction Monitoring; multipotent Mesenchymal Stem Cells; peripheral blood; platelet lysate
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Christou, I.; Mallis, P.; Michalopoulos, E.; Chatzistamatiou, T.; Mermelekas, G.; Zoidakis, J.; Vlahou, A.; Stavropoulos-Giokas, C. Evaluation of Peripheral Blood and Cord Blood Platelet Lysates in Isolation and Expansion of Multipotent Mesenchymal Stromal Cells. Bioengineering 2018, 5, 19.

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