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Bioengineering 2017, 4(2), 49; doi:10.3390/bioengineering4020049

Additive Manufacturing of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)/poly(ε-caprolactone) Blend Scaffolds for Tissue Engineering

BIOLab Research Group, Department of Chemistry and Industrial Chemistry, University of Pisa, UdR INSTM Pisa, via Moruzzi 13, 56124 Pisa, Italy
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Academic Editor: Martin Koller
Received: 27 April 2017 / Revised: 19 May 2017 / Accepted: 21 May 2017 / Published: 24 May 2017
(This article belongs to the Special Issue Advances in Polyhydroxyalkanoate (PHA) Production)
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Abstract

Additive manufacturing of scaffolds made of a polyhydroxyalkanoate blended with another biocompatible polymer represents a cost-effective strategy for combining the advantages of the two blend components in order to develop tailored tissue engineering approaches. The aim of this study was the development of novel poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)/ poly(ε-caprolactone) (PHBHHx/PCL) blend scaffolds for tissue engineering by means of computer-aided wet-spinning, a hybrid additive manufacturing technique suitable for processing polyhydroxyalkanoates dissolved in organic solvents. The experimental conditions for processing tetrahydrofuran solutions containing the two polymers at different concentrations (PHBHHx/PCL weight ratio of 3:1, 2:1 or 1:1) were optimized in order to manufacture scaffolds with predefined geometry and internal porous architecture. PHBHHx/PCL scaffolds with a 3D interconnected network of macropores and a local microporosity of the polymeric matrix, as a consequence of the phase inversion process governing material solidification, were successfully fabricated. As shown by scanning electron microscopy, thermogravimetric, differential scanning calorimetric and uniaxial compressive analyses, blend composition significantly influenced the scaffold morphological, thermal and mechanical properties. In vitro biological characterization showed that the developed scaffolds were able to sustain the adhesion and proliferation of MC3T3-E1 murine preosteoblast cells. The additive manufacturing approach developed in this study, based on a polymeric solution processing method avoiding possible material degradation related to thermal treatments, could represent a powerful tool for the development of customized PHBHHx-based blend scaffolds for tissue engineering. View Full-Text
Keywords: polyhydroxyalkanoates; poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); poly(ε-caprolactone); polymers blend; tissue engineering; scaffolds; additive manufacturing; computer-aided wet-spinning polyhydroxyalkanoates; poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); poly(ε-caprolactone); polymers blend; tissue engineering; scaffolds; additive manufacturing; computer-aided wet-spinning
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MDPI and ACS Style

Puppi, D.; Morelli, A.; Chiellini, F. Additive Manufacturing of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)/poly(ε-caprolactone) Blend Scaffolds for Tissue Engineering. Bioengineering 2017, 4, 49.

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