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Bioengineering 2016, 3(4), 31; doi:10.3390/bioengineering3040031

Focused Screening of ECM-Selective Adhesion Peptides on Cellulose-Bound Peptide Microarrays

1
Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Aichi 464-8601, Japan
2
Department of Biotechnology, Graduate School of Engineering, Nagoya University, Aichi 464-8603, Japan
3
Department of Cardiac Surgery, Nagoya University Graduate School of Medicine, Aichi 466-8550, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Danièle Noël
Received: 4 October 2016 / Revised: 14 November 2016 / Accepted: 16 November 2016 / Published: 19 November 2016
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Abstract

The coating of surfaces with bio-functional proteins is a promising strategy for the creation of highly biocompatible medical implants. Bio-functional proteins from the extracellular matrix (ECM) provide effective surface functions for controlling cellular behavior. We have previously screened bio-functional tripeptides for feasibility of mass production with the aim of identifying those that are medically useful, such as cell-selective peptides. In this work, we focused on the screening of tripeptides that selectively accumulate collagen type IV (Col IV), an ECM protein that accelerates the re-endothelialization of medical implants. A SPOT peptide microarray was selected for screening owing to its unique cellulose membrane platform, which can mimic fibrous scaffolds used in regenerative medicine. However, since the library size on the SPOT microarray was limited, physicochemical clustering was used to provide broader variation than that of random peptide selection. Using the custom focused microarray of 500 selected peptides, we assayed the relative binding rates of tripeptides to Col IV, collagen type I (Col I), and albumin. We discovered a cluster of Col IV-selective adhesion peptides that exhibit bio-safety with endothelial cells. The results from this study can be used to improve the screening of regeneration-enhancing peptides. View Full-Text
Keywords: peptide microarrays; extracellular matrix; collagen type IV; clustering; amino acid index; physicochemical property peptide microarrays; extracellular matrix; collagen type IV; clustering; amino acid index; physicochemical property
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kanie, K.; Kondo, Y.; Owaki, J.; Ikeda, Y.; Narita, Y.; Kato, R.; Honda, H. Focused Screening of ECM-Selective Adhesion Peptides on Cellulose-Bound Peptide Microarrays. Bioengineering 2016, 3, 31.

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