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Bioengineering 2016, 3(4), 23;

Horizontally Aligned Carbon Nanotube Based Biosensors for Protein Detection

School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore
Institute for Sports Research, Nanyang Technological University, Singapore 639798, Singapore
Department of Chemistry, Loughborough University, Loughborough LE11 3TU, UK
School of Engineering, Republic Polytechnic, Singapore 738984, Singapore
Department of Chemistry, Royal College of Science, Imperial College, London SW7 2AZ, UK
Author to whom correspondence should be addressed.
Academic Editor: Frank Alexis
Received: 3 August 2016 / Revised: 20 September 2016 / Accepted: 25 September 2016 / Published: 29 September 2016
(This article belongs to the Special Issue Nanoparticle Interaction with the Biological Milieu)
Full-Text   |   PDF [3531 KB, uploaded 29 September 2016]   |  


A novel horizontally aligned single-walled carbon nanotube (CNT) Field Effect Transistor (FET)-based biosensing platform for real-time and sensitive protein detections is proposed. Aligned nanotubes were synthesized on quartz substrate using catalyst contact stamping, surface-guided morphological growth and chemical vapor deposition gas-guided growth methods. Real-time detection of prostate-specific antigen (PSA) using as-prepared FET biosensors was demonstrated. The kinetic measurements of the biosensor revealed that the drain current (Id) decreased exponentially as the concentration of PSA increased, indicating that the proposed FET sensor is capable of quantitative protein detection within a detection window of up to 1 µM. The limit of detection (LOD) achieved by the proposed platform was demonstrated to be 84 pM, which is lower than the clinically relevant level (133 pM) of PSA in blood. Additionally, the reported aligned CNT biosensor is a uniform sensing platform that could be extended to real-time detections of various biomarkers. View Full-Text
Keywords: horizontal alignment; CNT-based biosensor; protein detection horizontal alignment; CNT-based biosensor; protein detection

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Chen, H.; Huang, J.; Fam, D.W.H.; Tok, A.I.Y. Horizontally Aligned Carbon Nanotube Based Biosensors for Protein Detection. Bioengineering 2016, 3, 23.

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