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Bioengineering 2016, 3(2), 14; doi:10.3390/bioengineering3020014

Biodiesel Production by Aspergillus niger Lipase Immobilized on Barium Ferrite Magnetic Nanoparticles

1
Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Cairo 11371, Egypt
2
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11371, Egypt
3
Chemical Engineering Department, Military Technical College, Cairo 11371, Egypt
*
Author to whom correspondence should be addressed.
Academic Editor: Anthony Guiseppi-Elie
Received: 19 January 2016 / Revised: 30 April 2016 / Accepted: 6 May 2016 / Published: 12 May 2016
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Abstract

In this study, Aspergillus niger ADM110 fungi was gamma irradiated to produce lipase enzyme and then immobilized onto magnetic barium ferrite nanoparticles (BFN) for biodiesel production. BFN were prepared by the citrate sol-gel auto-combustion method and characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared (FTIR) and scanning electron microscopy with energy dispersive analysis of X-ray (SEM/EDAX) analysis. The activities of free and immobilized lipase were measured at various pH and temperature values. The results indicate that BFN–Lipase (5%) can be reused in biodiesel production without any treatment with 17% loss of activity after five cycles and 66% loss in activity in the sixth cycle. The optimum reaction conditions for biodiesel production from waste cooking oil (WCO) using lipase immobilized onto BFN as a catalyst were 45 °C, 4 h and 400 rpm. Acid values of WCO and fatty acid methyl esters (FAMEs) were 1.90 and 0.182 (mg KOH/g oil), respectively. The measured flash point, calorific value and cetane number were 188 °C, 43.1 MJ/Kg and 59.5, respectively. The cloud point (−3 °C), pour point (−9 °C), water content (0.091%) and sulfur content (0.050%), were estimated as well. View Full-Text
Keywords: biodiesel; Aspergillus niger; lipase immobilization; barium ferrite magnetic nanoparticles; fatty acid methyl ester biodiesel; Aspergillus niger; lipase immobilization; barium ferrite magnetic nanoparticles; fatty acid methyl ester
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

El-Batal, A.I.; Farrag, A.A.; Elsayed, M.A.; El-Khawaga, A.M. Biodiesel Production by Aspergillus niger Lipase Immobilized on Barium Ferrite Magnetic Nanoparticles. Bioengineering 2016, 3, 14.

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