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Bioengineering 2015, 2(1), 2-14; doi:10.3390/bioengineering2010002

Label Free Detection of L-Glutamate Using Microfluidic Based Thermal Biosensor

1
The Center for Biomedical Engineering and Rehabilitation Science, Louisiana Tech University, Ruston, LA 71272, USA
2
Department of Mechanical & Industrial Engineering, Qatar University, P.O. Box 2713, Doha, Qatar
*
Author to whom correspondence should be addressed.
Academic Editor: Anthony Guiseppi-Elie
Received: 12 November 2014 / Accepted: 7 January 2015 / Published: 12 January 2015
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Abstract

A thermoelectric biosensor for the detection of L-glutamate concentration was developed. The thermoelectric sensor is integrated into a micro-calorimeter which measures the heat produced by biochemical reactions. The device contains a single flow channel that is 120 µm high and 10 mm wide with two fluid inlets and one fluid outlet. An antimony-bismuth (Sb-Bi) thermopile with high common mode rejection ratio is attached to the lower channel wall and measures the dynamic changes in the temperature when L-glutamate undergoes oxidative deamination in the presence of glutamate oxidase (GLOD). The thermopile has a Seebeck coefficient of ~7 µV·(m·K)−1. The device geometry, together with hydrodynamic focusing, eliminates the need of extensive temperature control. Layer-by-layer assembly is used to immobilize GLOD on the surface of glass coverslips by alternate electrostatic adsorption of polyelectrolyte and GLOD. The impulse injection mode using a 6-port injection valve minimizes sample volume to 5 µL. The sensitivity of the sensor for glutamate is 17.9 nVs·mM−1 in the linear range of 0–54 mM with an R2 value of 0.9873. The lowest detection limit of the sensor for glutamate is 5.3 mM. View Full-Text
Keywords: thermoelectric; label-free; biosensor; L-glutamate; L-glutamate oxidase; layer-by-layer self-assembly thermoelectric; label-free; biosensor; L-glutamate; L-glutamate oxidase; layer-by-layer self-assembly
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kopparthy, V.L.; Tangutooru, S.M.; Guilbeau, E.J. Label Free Detection of L-Glutamate Using Microfluidic Based Thermal Biosensor. Bioengineering 2015, 2, 2-14.

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