Label Free Detection of L-Glutamate Using Microfluidic Based Thermal Biosensor
AbstractA thermoelectric biosensor for the detection of L-glutamate concentration was developed. The thermoelectric sensor is integrated into a micro-calorimeter which measures the heat produced by biochemical reactions. The device contains a single flow channel that is 120 µm high and 10 mm wide with two fluid inlets and one fluid outlet. An antimony-bismuth (Sb-Bi) thermopile with high common mode rejection ratio is attached to the lower channel wall and measures the dynamic changes in the temperature when L-glutamate undergoes oxidative deamination in the presence of glutamate oxidase (GLOD). The thermopile has a Seebeck coefficient of ~7 µV·(m·K)−1. The device geometry, together with hydrodynamic focusing, eliminates the need of extensive temperature control. Layer-by-layer assembly is used to immobilize GLOD on the surface of glass coverslips by alternate electrostatic adsorption of polyelectrolyte and GLOD. The impulse injection mode using a 6-port injection valve minimizes sample volume to 5 µL. The sensitivity of the sensor for glutamate is 17.9 nVs·mM−1 in the linear range of 0–54 mM with an R2 value of 0.9873. The lowest detection limit of the sensor for glutamate is 5.3 mM. View Full-Text
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Kopparthy, V.L.; Tangutooru, S.M.; Guilbeau, E.J. Label Free Detection of L-Glutamate Using Microfluidic Based Thermal Biosensor. Bioengineering 2015, 2, 2-14.
Kopparthy VL, Tangutooru SM, Guilbeau EJ. Label Free Detection of L-Glutamate Using Microfluidic Based Thermal Biosensor. Bioengineering. 2015; 2(1):2-14.Chicago/Turabian Style
Kopparthy, Varun L.; Tangutooru, Siva M.; Guilbeau, Eric J. 2015. "Label Free Detection of L-Glutamate Using Microfluidic Based Thermal Biosensor." Bioengineering 2, no. 1: 2-14.