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Bioengineering 2014, 1(4), 231-245; doi:10.3390/bioengineering1040231

Characterization and Application of a Disposable Rotating Bed Bioreactor for Mesenchymal Stem Cell Expansion

1
Department for Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria
2
Institute for Technical Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstrasse 5, 30167 Hanover, Germany
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Christoph Herwig
Received: 11 September 2014 / Revised: 18 November 2014 / Accepted: 25 November 2014 / Published: 27 November 2014
(This article belongs to the Special Issue Biopharmaceutical Process Development)
View Full-Text   |   Download PDF [7164 KB, uploaded 27 November 2014]   |  

Abstract

Recruitment of mesenchymal stromal cells (MSC) into the field of tissue engineering is a promising development since these cells can be expanded vivo to clinically relevant numbers and, after expansion, retain their ability to differentiate into various cell lineages. Safety requirements and the necessity to obtain high cell numbers without frequent subcultivation of cells raised the question of the possibility of expanding MSC in one-way (single-use) disposable bioreactors. In this study, umbilical cord-derived MSC (UC-MSC) were expanded in a disposable Z 2000 H bioreactor under dynamic conditions. Z was characterized regarding residence time and mixing in order to evaluate the optimal bioreactor settings, enabling optimal mass transfer in the absence of shear stress, allowing an reproducible expansion of MSC, while maintaining their stemness properties. Culture of the UC-MSC in disposable Z 2000 H bioreactor resulted in a reproducible 8-fold increase of cell numbers after 5 days. Cells were shown to maintain specific MSC surface marker expression as well as trilineage differentiation potential and lack stress-induced premature senescence. View Full-Text
Keywords: umbilical cord stem cells; MSC expansion; dynamic culture; bioreactor umbilical cord stem cells; MSC expansion; dynamic culture; bioreactor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Neumann, A.; Lavrentieva, A.; Heilkenbrinker, A.; Loenne, M.; Kasper, C. Characterization and Application of a Disposable Rotating Bed Bioreactor for Mesenchymal Stem Cell Expansion. Bioengineering 2014, 1, 231-245.

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