Dynamic Experiments for Bioprocess Parameter Optimization with Extreme Halophilic Archaea
Abstract
:1. Introduction
1.1. Halophiles
1.2. Bioreactor Technology with Extreme Halophiles
1.3. Optimal Process Parameters for Extreme Halophilic Archaea
1.4. Dynamic Experiments for Process Parameter Optimization
1.5. Goal and Structure
2. Experimental Section
2.1. Strains and Cultivation Procedures
2.2. Defined Medium Composition
2.3. Bioreactor Setup, Cultivation Conditions
2.4. Experimental Design
2.5. Off-Line Analytics
2.6. Data Evaluation
3. Results and Discussion
3.1. Ramp Design
3.2. Analytics: The Necessity for Off-Line Data
3.3. Ramp Down
3.4. Ramp Up
3.5. Multivariate Data Analysis: Supporting the Findings of Temperature and pH Optima
3.6. Verification of the Dynamic Experimental Results
dynamic conditions | steady state | |||||
---|---|---|---|---|---|---|
Experiment | down 1 | down 2 | up 1 | up 2 | continuous culture | batch culture |
T (°C) | 37–36 | 38–36 | 38–39 | 36–37 | 37 | 37 |
pH | 7.2 | 7.2 | 7.2 | 7.2 | 7.2 | 7.2 |
D = µ (h−1) | 0.030 ± 0.005 | 0.035 ± 0.003 | µmax | |||
rGly (g/L/h) | 0.104 | 0.071 | 0.076 | 0.057 | 0.120 | 0.108 |
rAce (g/L/h) | 0.003 | 0.008 | 0.005 | 0.004 | 0.002 | 0.002 |
rOD (g/L/h) | 0.080 | 0.052 | 0.067 | 0.049 | 0.070 | 0.083 |
qS (g/g/h) | 0.056 ± 0.005 | 0.050 ± 0.005 | 0.051 ± 0.005 | 0.053 ± 0.005 | 0.047 ± 0.005 | 0.054 ± 0.005 |
3.7. Data Repeatability and Reproducibility
4. Conclusions
Conflicts of Interest
References
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Lorantfy, B.; Seyer, B.; Herwig, C. Dynamic Experiments for Bioprocess Parameter Optimization with Extreme Halophilic Archaea. Bioengineering 2014, 1, 1-17. https://doi.org/10.3390/bioengineering1010001
Lorantfy B, Seyer B, Herwig C. Dynamic Experiments for Bioprocess Parameter Optimization with Extreme Halophilic Archaea. Bioengineering. 2014; 1(1):1-17. https://doi.org/10.3390/bioengineering1010001
Chicago/Turabian StyleLorantfy, Bettina, Bernhard Seyer, and Christoph Herwig. 2014. "Dynamic Experiments for Bioprocess Parameter Optimization with Extreme Halophilic Archaea" Bioengineering 1, no. 1: 1-17. https://doi.org/10.3390/bioengineering1010001