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ChemEngineering 2017, 1(1), 6; doi:10.3390/chemengineering1010006

Effect of Methyl β-cyclodextrin on Radical Scavenging Kinetics of Olive Leaf Extracts and Interactions with Ascorbic Acid

1
School of Environment, University of the Aegean, Lesbos 81400, Greece
2
Department of Food Technology, Technological Educational Institute (T.E.I.) of Thessaly, Karditsa 43100, Greece
*
Author to whom correspondence should be addressed.
Received: 25 August 2017 / Revised: 6 September 2017 / Accepted: 7 September 2017 / Published: 11 September 2017
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Abstract

Olive leaf (OLL) extracts contain a high load of antioxidant polyphenols with significant pharmacological potency. In this study, the use of a novel natural deep eutectic solvent enabled the effective extraction of OLL polyphenols and their testing as radical scavengers, in the presence or absence of methyl β-cyclodextrin (m-β-CD), using descriptive kinetics. Testing was extended to include interactions with ascorbic acid—a natural powerful antioxidant—by implementing a response surface methodology. The kinetic study showed that m-β-CD may hinder the radical scavenging effect of OLL extracts, yielding lower stoichiometry upon reaction with the radical probe 2,2-diphenyl-1-picrylhydrazy (DPPH). The extension of the reaction time to determine the total stoichiometry confirmed this effect. As a further concurrence, the interactions of OLL extracts with ascorbic acid showed lower radical scavenging performance in the presence of m-β-CD. These results were discussed on the grounds of the role that m-β-CD may play in similar systems. View Full-Text
Keywords: antioxidants; deep eutectic solvents; methyl β-cyclodextrin; olive leaf extracts; polyphenols antioxidants; deep eutectic solvents; methyl β-cyclodextrin; olive leaf extracts; polyphenols
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Athanasiadis, V.; Lalas, S.; Makris, D.P. Effect of Methyl β-cyclodextrin on Radical Scavenging Kinetics of Olive Leaf Extracts and Interactions with Ascorbic Acid. ChemEngineering 2017, 1, 6.

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