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Genotoxicity of Silver Nanoparticles in Lung Cells of Sprague Dawley Rats after 12 Weeks of Inhalation Exposure
Bioconvergence Department, Korea Conformity Laboratories, Incheon 406-840, Korea
Center for Environment, Health and Welfare Research, Korea Institute of Science and Technology, Seoul 136-791, Korea
Institute of Nanoproduct Safety Research, Hoseo University, 165 Sechul-ri, Baebang-myun, Asan 336-795, Korea
Department of Mechanical Engineering, Hanyang University, Seoul 133-791, Korea
* Author to whom correspondence should be addressed.
Received: 3 October 2013; in revised form: 6 November 2013 / Accepted: 7 November 2013 / Published: 19 November 2013
Abstract: Due to the widespread use of silver nanoparticles in consumer products, the toxicity of silver nanoparticles has also been studied in relation to their application. However, most genotoxicity studies of silver nanoparticles have been performed in vitro. Therefore, this study evaluated the DNA damage to lung cells caused by repeated inhalation of silver nanoparticles. Male Sprague Dawley rats were exposed to silver nanoparticles for 12 weeks in a whole-body inhalation chamber. The animals were divided into one control group and three dose groups that were exposed to silver nanoparticles (14–15 nm diameter) at concentrations of 0.66 × 106 particles/cm3 (49 μg/m3, low dose), 1.41 × 106 particles/cm3 (117 μg/m3, middle dose), and 3.24 × 106 particles /cm3 (381 μg/m3, high dose), respectively, for six hours/day over 12 weeks. The rats were sacrificed after the 12-week exposure period and the DNA damage assessed using a Comet assay of cells obtained from the right lungs. The olive tail moment values were 2.93 ± 0.19, 3.81 ± 0.23, 3.40 ± 0.22, and 5.16 ± 0.32 for the control, low-, middle-, and high-dose groups, respectively. Although no dose-dependent results were observed, a significant increase in the level of DNA damage was noted for the high-dose group.
Keywords: silver nanoparticle; lung cells; genotoxicity; comet assay; single cell gel electrophoresis; inhalation exposure
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Cho, H.S.; Sung, J.H.; Song, K.S.; Kim, J.S.; Ji, J.H.; Lee, J.H.; Ryu, H.R.; Ahn, K.; Yu, I.J. Genotoxicity of Silver Nanoparticles in Lung Cells of Sprague Dawley Rats after 12 Weeks of Inhalation Exposure. Toxics 2013, 1, 36-45.
Cho HS, Sung JH, Song KS, Kim JS, Ji JH, Lee JH, Ryu HR, Ahn K, Yu IJ. Genotoxicity of Silver Nanoparticles in Lung Cells of Sprague Dawley Rats after 12 Weeks of Inhalation Exposure. Toxics. 2013; 1(1):36-45.
Cho, Hyun S.; Sung, Jae H.; Song, Kyung S.; Kim, Jin S.; Ji, Jun H.; Lee, Ji H.; Ryu, Hyeon R.; Ahn, Kangho; Yu, Il J. 2013. "Genotoxicity of Silver Nanoparticles in Lung Cells of Sprague Dawley Rats after 12 Weeks of Inhalation Exposure." Toxics 1, no. 1: 36-45.