Next Article in Journal
In Situ Raman Analysis of CO2—Assisted Drying of Fruit-Slices
Previous Article in Journal
Bioactive Peptides in Animal Food Products
Article Menu

Export Article

Open AccessArticle
Foods 2017, 6(5), 36; doi:10.3390/foods6050036

Molecular Surveillance of Cronobacter spp. Isolated from a Wide Variety of Foods from 44 Different Countries by Sequence Typing of 16S rRNA, rpoB and O-Antigen Genes

1
Southeast Regional Laboratory, U.S. Food and Drug Administration, Atlanta, GA 30309, USA
2
Division of Epidemiology, Biostatistics, and Environmental Health, School of Public Health, University of Memphis, Memphis, TN 38152, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Debabrata Biswas
Received: 4 April 2017 / Revised: 5 May 2017 / Accepted: 8 May 2017 / Published: 11 May 2017
View Full-Text   |   Download PDF [650 KB, uploaded 11 May 2017]   |  

Abstract

Cronobacter spp. are emerging infectious bacteria that can cause acute meningitis and necrotizing enterocolitis in neonatal and immunocompromised individuals. Although this opportunistic human-pathogenic microorganism has been isolated from a wide variety of food and environmental samples, it has been primarily linked to foodborne outbreaks associated with powdered infant formula. The U.S. Food and Drug Administration use the presence of these microbes as one of the criteria to assess food adulteration and to implement regulatory actions. In this study, we have examined 195 aliquots of enrichments from the nine major categories of foods (including baby and medical food, dairy products, dried food, frozen food, pet food, produce, ready-to-eat snacks, seafood, and spices) from 44 countries using conventional microbiological and molecular techniques. The typical colonies of Cronobacter were then identified by VITEK2 and real-time PCR. Subsequently, sequence typing was performed on the 51 recovered Cronobacter isolates at the 16S rRNA, rpoB and seven O-antigen loci for species identification in order to accomplish an effective surveillance program for the control and prevention of foodborne illnesses. View Full-Text
Keywords: Cronobacter spp.; isolation; chromogenic and traditional media; molecular typing; foodborne disease Cronobacter spp.; isolation; chromogenic and traditional media; molecular typing; foodborne disease
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Miranda, N.; Banerjee, P.; Simpson, S.; Kerdahi, K.; Sulaiman, I.M. Molecular Surveillance of Cronobacter spp. Isolated from a Wide Variety of Foods from 44 Different Countries by Sequence Typing of 16S rRNA, rpoB and O-Antigen Genes. Foods 2017, 6, 36.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Foods EISSN 2304-8158 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top