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Foods 2015, 4(3), 318-327; doi:10.3390/foods4030318

Use of Phytone Peptone to Optimize Growth and Cell Density of Lactobacillus reuteri

1
Food Microbiology and Biotechnology Laboratory, Agricultural and Technical State University, 173 Carver Hall, Greensboro, NC 27411, USA
2
Department of Exercise Physiology, College of Sport Sciences and Physical Activity King Saud University, P.O. Box 2458, Riyadh, 11451, Saudi Arabia
*
Author to whom correspondence should be addressed.
Academic Editor: Felix Barron
Received: 4 March 2015 / Revised: 25 June 2015 / Accepted: 24 July 2015 / Published: 10 August 2015
(This article belongs to the Special Issue Fermented Foods and Probiotics)
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Abstract

The objective of this study was to determine the use of phytone peptone to optimize the growth and cell density of Lactobacillus reuteri. Four strains of L. reuteri (DSM 20016, SD 2112, CF 2-7F, and MF 2-3,) were used in this study. An overnight culture of individual strains was inoculated into fresh basal media with various protein sources (peptone, tryptone, proteose peptone #3, phytone peptone, tryptic soy broth, yeast extract, and beef extract). Samples were then mixed well and incubated at 37 °C for 15 h. Bacterial growth was monitored by measuring turbidity (optical density 610 nm) at different time intervals during the incubation period. At the end of incubation, samples were plated on de-Man Rogosa Sharpe (MRS) agar to determine the bacterial population. Our results showed that phytone peptone promoted the growth of L. reuteri (p < 0.05) by 1.4 log CFU/mL on average compared to the control samples. Therefore, phytone peptone could be included in laboratory media to enhance growth and increase the cell density of L. reuteri. View Full-Text
Keywords: cell density; growth; L. reuteri; phytone peptone; protein cell density; growth; L. reuteri; phytone peptone; protein
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Atilola, O.A.; Gyawali, R.; Aljaloud, S.O.; Ibrahim, S.A. Use of Phytone Peptone to Optimize Growth and Cell Density of Lactobacillus reuteri. Foods 2015, 4, 318-327.

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