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Photonics 2017, 4(2), 32; doi:10.3390/photonics4020032

Photothermal Microscopy for High Sensitivity and High Resolution Absorption Contrast Imaging of Biological Tissues

1
Faculty of Systems Engineering, Wakayama-University, Wakayama 640-8510, Japan
2
Advanced Ultrafast Laser Research Center, The University of Electro-Communications, 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan
3
JST, CREST, K’ Gobancho 7, Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan
4
Department of Electrophysics, National Chiao-Tung University, Hsinchu 300, Taiwan
5
Institute of Laser Engineering, Osaka University, 2-6 Yamada-oka, Suita, Osaka 565-0971, Japan
*
Author to whom correspondence should be addressed.
Received: 13 March 2017 / Revised: 4 April 2017 / Accepted: 13 April 2017 / Published: 19 April 2017
(This article belongs to the Special Issue Superresolution Optical Microscopy)
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Abstract

Photothermal microscopy is useful to visualize the distribution of non-fluorescence chromoproteins in biological specimens. Here, we developed a high sensitivity and high resolution photothermal microscopy with low-cost and compact laser diodes as light sources. A new detection scheme for improving signal to noise ratio more than 4-fold is presented. It is demonstrated that spatial resolution in photothermal microscopy is up to nearly twice as high as that in the conventional widefield microscopy. Furthermore, we demonstrated the ability for distinguishing or identifying biological molecules with simultaneous muti-wavelength imaging. Simultaneous photothermal and fluorescence imaging of mouse brain tissue was conducted to visualize both neurons expressing yellow fluorescent protein and endogenous non-fluorescent chromophores. View Full-Text
Keywords: photothermal effect; pump-probe microscopy; superresolution photothermal effect; pump-probe microscopy; superresolution
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Miyazaki, J.; Kobayahsi, T. Photothermal Microscopy for High Sensitivity and High Resolution Absorption Contrast Imaging of Biological Tissues. Photonics 2017, 4, 32.

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