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Separations 2017, 4(4), 37; https://doi.org/10.3390/separations4040037

Comparison of Separation of Seed Oil Triglycerides Containing Isomeric Conjugated Octadecatrienoic Acid Moieties by Reversed-Phase HPLC

1
Institute of Engineering Technologies and Natural Sciences of Belgorod National Research University, Pobeda str. 85, Belgorod 308015, Russia
2
Pharmaceutical Department of of Buon Ma Thuot University, 298 Ha Huy Tap, Buon Ma Thuot, Daclak 630000, Vietnam
*
Author to whom correspondence should be addressed.
Received: 8 November 2017 / Revised: 23 November 2017 / Accepted: 3 December 2017 / Published: 8 December 2017
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Abstract

Relative retention analysis and increment approach were applied for the comparison of triglycerides (TGs) retention of a broad set of plant seed oils with isomeric conjugated octadecatrienoic acids (CLnA) by reversed-phase HPLC for “propanol-2-acetonitrile” mobile phases and Kromasil 100-5C18 stationary phase with diode array detection (DAD) and mass spectrometric (MS) detection. The subjects of investigation were TGs of seed oils: Calendula officinalis, Catalpa ovata, Jacaranda mimosifolia, Centranthus ruber, Momordica charantia, Trichosanthes anguina, Punica granatum, Thladiantha dubia, Valeriana officinalis, and Vernicia montana. It was found that a sequence of elution of TGs of the same types is the same without any inversions for full range of mobile phase compositions: punicic (C18:39Z11E13Z) < jacaric (C18:38Z10E12Z) < catalpic (C18:39E11E13Z) < α-eleostearic (C18:39Z11E13E) < calendic (C18:38E10E12Z) < β-eleostearic (C18:39E11E13E) < all-E calendic (C18:38E10E12E) acids. TGs and fatty acid compositions were calculated for all oil samples. Regularities of solute retentions as a function of isomeric conjugated octadecatrienoic acid moiety structure are discussed. Thus, it was proven that it is possible to differentiate TGs of complex composition with moieties of all natural CLnA by retention control accomplished by electronic spectra comparison, even though there are only three types of electronic-vibration spectra for seven isomeric CLnA. View Full-Text
Keywords: reversed-phase HPLC; seed oil; conjugated octadecatrienoic acids; increment approach; relative retention analysis reversed-phase HPLC; seed oil; conjugated octadecatrienoic acids; increment approach; relative retention analysis
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Van Nguyen, A.; Deineka, V.; Deineka, L.; Vu Thi Ngoc, A. Comparison of Separation of Seed Oil Triglycerides Containing Isomeric Conjugated Octadecatrienoic Acid Moieties by Reversed-Phase HPLC. Separations 2017, 4, 37.

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