Next Article in Journal
Recent Advances in Microextraction Techniques of Antipsychotics in Biological Fluids Prior to Liquid Chromatography Analysis
Previous Article in Journal
Determination of Posaconazole in Plasma/Serum by High-Performance Liquid Chromatography with Fluorescence Detection
Article Menu
Issue 2 (June) cover image

Export Article

Open AccessArticle
Separations 2017, 4(2), 17; doi:10.3390/separations4020017

LC-MS as a Tool to Overcome the Limitations of Self-Reported Dietary Assessments in the Determination of Wine Intake

Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Veterinary Science, Universidade de Santiago de Compostela, 27002 Lugo, Spain
Author to whom correspondence should be addressed.
Academic Editor: Fabio Gosetti
Received: 31 January 2017 / Revised: 3 May 2017 / Accepted: 4 May 2017 / Published: 10 May 2017
View Full-Text   |   Download PDF [534 KB, uploaded 10 May 2017]   |  


The nutritional assessment of individuals is usually performed using highly subjective data collecting tools such as food frequency questionnaires, dietary recalls and food records. However, people are not always capable of recalling all foods (and ingredients) consumed, and in some cases, the intake of specific foods is intentionally omitted. Even though wine is considered positive for cardiovascular status, and is an essential part of Mediterranean culture, individuals may not always report its consumption accurately due to the existence of social preconceptions about alcoholic beverages. In this study, the presence of free resveratrol has been determined in human plasma from 25 Spanish volunteers using liquid chromatography coupled to mass spectrometry (LC-MS). This phenolic compound proved to be useful as a dietary biomarker for wine intake in a known population, and the results were compared with those obtained by self-reported dietary assessments. However, certain limitations must also be taken into account such as inter-individual variations and the type of wine consumed. The LC-MS method was validated for trans-resveratrol determination in human plasma, with an LOD (limit of detection) of 50 ng·mL−1 and an LOQ (limit of quantification) of 150 ng·mL−1, respectively. View Full-Text
Keywords: resveratrol; LC; MS; biomarker; wine; dietary intake; FFQ; self-reported resveratrol; LC; MS; biomarker; wine; dietary intake; FFQ; self-reported

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Regal, P.; Porto-Arias, J.J.; Lamas, A.; Paz, L.; Barreiro, F.; Cepeda, A. LC-MS as a Tool to Overcome the Limitations of Self-Reported Dietary Assessments in the Determination of Wine Intake. Separations 2017, 4, 17.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics



[Return to top]
Separations EISSN 2297-8739 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top