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Separations 2017, 4(2), 10; doi:10.3390/separations4020010

Optimization of Lactoperoxidase and Lactoferrin Separation on an Ion-Exchange Chromatography Step

1
Department of Chemical and Biochemical Engineering, University of Western Ontario, London, ON N6A 5B9, Canada
2
Faculty of Engineering and Applied Science, Memorial University of Newfoundland, St John’s, NL A1B 3X9, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: Christopher R. Harrison
Received: 11 January 2017 / Revised: 13 March 2017 / Accepted: 17 March 2017 / Published: 23 March 2017
(This article belongs to the Special Issue Ion Chromatography)
View Full-Text   |   Download PDF [3243 KB, uploaded 23 March 2017]   |  

Abstract

Lactoperoxidase (LP), which is a high-value minor whey protein, has recently drawn extensive attention from research scientists and industry due to its multiplicity of function and potential therapeutic applications. In this study, the separation and optimization of two similar-sized proteins, LP and lactoferrin (LF) were investigated using strong cation exchange column chromatography. A two-step optimization strategy was developed for the separation of LP and LF. Optimization was started with central composite design-based experiments to characterize the influences of different decision variables, namely, flow rate, length of gradient, and final salt concentration in the linear elution gradient step on the yield of LP. This was followed by a more accurate optimization of ion-exchange chromatography (IEC) separation of LP and LF based on an experimentally verified chromatographic model. The optimal operating points were found and the results were compared with validation experiments. Predictions respecting yield confirmed a very good agreement with experimental results with improved product purity. View Full-Text
Keywords: cation exchange chromatography; minor milk protein; response surface modeling; steric mass action (SMA) isotherm; optimization of separation conditions cation exchange chromatography; minor milk protein; response surface modeling; steric mass action (SMA) isotherm; optimization of separation conditions
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Faraji, N.; Zhang, Y.; Ray, A.K. Optimization of Lactoperoxidase and Lactoferrin Separation on an Ion-Exchange Chromatography Step. Separations 2017, 4, 10.

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