Next Article in Journal
Simultaneous Determination of Daidzein, Genistein and Formononetin in Coffee by Capillary Zone Electrophoresis
Next Article in Special Issue
Nano-Doped Monolithic Materials for Molecular Separation
Previous Article in Journal
HPLC-UV Polyphenolic Profiles in the Classification of Olive Oils and Other Vegetable Oils via Principal Component Analysis
Previous Article in Special Issue
Monoliths in Bioprocess Technology
Article Menu

Export Article

Open AccessCommunication
Separations 2016, 3(4), 34; doi:10.3390/separations3040034

In Situ Measurement of Polymer Layer Thickness in Porous Layer Open Tubular (PLOT) Columns Using Optical Absorbance in the Near-IR Range

1
National Centre for Sensor Research, Dublin City University, Collins Avenue, Glasnevin, Dublin 9, Ireland
2
School of Biotechnology, Dublin City University, Collins Avenue, Glasnevin, Dublin 9, Ireland
3
Australian Centre for Research on Separation Science, University of Tasmania, Hobart TAS 7001, Australia
*
Author to whom correspondence should be addressed.
Academic Editor: Zuzana Zajickova
Received: 8 September 2016 / Revised: 16 November 2016 / Accepted: 6 December 2016 / Published: 12 December 2016
(This article belongs to the Special Issue Monolithic Columns in Separation Sciences)
View Full-Text   |   Download PDF [2329 KB, uploaded 12 December 2016]   |  

Abstract

Highly reproducible fabrication of porous layer open tubular (PLOT) structures in fused silica capillaries is often challenging; thus, methods to measure layer thickness growth in real time represent a powerful tool for the production of such columns. The work presented herein demonstrates the application of optical absorbance in the near-infrared (near IR) range for the in-process measurement of polymer layer growth inside fused silica capillaries during the fabrication of PLOT columns. The proposed technique can be used for both on- and off-line measurements of layer thickness for thermal- and photo- initiated polymerisation methods, performed in either polytetrafluoroethylene (PTFE)- or polyimide-coated capillaries. Measurements of layer thickness were carried out at λ 700 nm, using 100 μm and 8 μm optical fibres, yielding relative standard deviation (%RSD) values of 27% and 22%, respectively. View Full-Text
Keywords: PLOT columns; polymerisation; in-process layer thickness measurement PLOT columns; polymerisation; in-process layer thickness measurement
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Collins, D.; Nesterenko, E.; Paull, B. In Situ Measurement of Polymer Layer Thickness in Porous Layer Open Tubular (PLOT) Columns Using Optical Absorbance in the Near-IR Range. Separations 2016, 3, 34.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Separations EISSN 2297-8739 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top