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Separations 2016, 3(2), 17; doi:10.3390/separations3020017

Minor Wheat Protein Fractions Analysis by Using Capillary Zone Electrophoresis

Istituto di Bioscienze e Biorisorse, Consiglio Nazionale delle Ricerche (IBBR-CNR), via Amendola 165/a, 70126 Bari, Italy
Academic Editor: Timothy Strein
Received: 18 January 2016 / Revised: 18 May 2016 / Accepted: 24 May 2016 / Published: 3 June 2016
(This article belongs to the Special Issue Electrophoretic Analyses in Capillaries and Microfluidic Devices)
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Abstract

The wheat proteins soluble in chloroform-methanol mixtures are associated with several kinds of food allergies. A separation method based on capillary zone electrophoresis (CZE) with UV detection was developed for the analysis of these mixtures. An acidic phosphoric acid/β-alanine (pH 2.5) buffer containing HPMC, urea and acetonitrile was used for the separation. The capillary electrophoresis (CE) was able to complete the analysis in six minutes. The electrophoregrams of extracts of both durum and common wheat commercial cultivars were compared. The registered cultivar (cv.) Kamut® was included as a representative of rustic cereal species. A different number of peaks were detected in the profile relative to the tetraploid and exaploid analyzed cultivars. Three main peaks were observed for all tetraploid cultivars, while four peaks were detected for the common wheat cultivars. The peak corresponding to the α-amylase inhibitor type III was identified in the common wheat electrophoregram. The possibility of quantitative determination of this inhibitor has been investigated. View Full-Text
Keywords: alpha-amylase inhibitors; capillary zone electrophoresis; protein selectively extracted with chloroform/methanol (CM-proteins); commercial cultivars; Triticum species (spp.); wheat allergens alpha-amylase inhibitors; capillary zone electrophoresis; protein selectively extracted with chloroform/methanol (CM-proteins); commercial cultivars; Triticum species (spp.); wheat allergens
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Piergiovanni, A.R. Minor Wheat Protein Fractions Analysis by Using Capillary Zone Electrophoresis. Separations 2016, 3, 17.

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