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Biomedicines 2017, 5(4), 70; https://doi.org/10.3390/biomedicines5040070

Comparative Therapeutic Effects of Plant-Extract Synthesized and Traditionally Synthesized Gold Nanoparticles on Alcohol-Induced Inflammatory Activity in SH-SY5Y Cells In Vitro

Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Twin-Cities campus, St Paul, MN 55108, USA
Received: 21 November 2017 / Revised: 4 December 2017 / Accepted: 7 December 2017 / Published: 15 December 2017
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Abstract

The present study describes potential beneficial and adverse effects of plant-extract synthesized gold nanoparticles (AuNPs) on ethanol toxicity in SH-SY5Y cells. Although kudzu root extract (K), edible-gum extract (G), alone or in combination (KG), reduced Au3+ into AuNPs, the extract’s composition and the reaction temperature determined their size (AuNPKG(90<50<37) << AuNPK (90,50<37) < AuNPG (90<50); the subscript KG, K, or G is extract identification and numerical vales are reaction temperature in Celsius) and biological properties (AuNPKG (90,50>37) << AuNPK (90,50>37) < AuNPG (90,50)). The surface of each AuNP contained the extract’s active ingredients, that were analyzed and confirmed using laser desorption ionization (LDI)) and low-matrix laser desorption-ionization (LMALDI). AuNPKG-50 was (i) least toxic to SH-SY5Y cells, but most effective in suppressing the adverse effects of ethanol on SH-SY5Y cells, and (ii) more effective than a combination of free kudzu and gum extracts. The beneficial and adverse effects of AuNPs may have been modified by the formation of proteins corona. This study provides a proof of concept for possible application of plant-extract synthesized AuNPs in mitigating ethanol toxicity. View Full-Text
Keywords: ethanol; gold nanoparticles; plant extracts; kudzu root; edible gum; cell viability; apoptosis; necrosis; lactate dehydrogenase; lipid peroxidase ethanol; gold nanoparticles; plant extracts; kudzu root; edible gum; cell viability; apoptosis; necrosis; lactate dehydrogenase; lipid peroxidase
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Singh, A.K. Comparative Therapeutic Effects of Plant-Extract Synthesized and Traditionally Synthesized Gold Nanoparticles on Alcohol-Induced Inflammatory Activity in SH-SY5Y Cells In Vitro. Biomedicines 2017, 5, 70.

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