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Biomedicines 2017, 5(3), 49; doi:10.3390/biomedicines5030049

Aptamer Cell-Based Selection: Overview and Advances

Istituto di Endocrinologia ed Oncologia Sperimentale “G. Salvatore”, CNR, Naples 80100, Italy
These authors contributed equally to this work.
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Received: 12 July 2017 / Revised: 3 August 2017 / Accepted: 8 August 2017 / Published: 14 August 2017
(This article belongs to the Special Issue Engineering Aptamers for Biomedical Applications)
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Abstract

Aptamers are high affinity single-stranded DNA/RNA molecules, produced by a combinatorial procedure named SELEX (Systematic Evolution of Ligands by Exponential enrichment), that are emerging as promising diagnostic and therapeutic tools. Among selection strategies, procedures using living cells as complex targets (referred as “cell-SELEX”) have been developed as an effective mean to generate aptamers for heavily modified cell surface proteins, assuring the binding of the target in its native conformation. Here we give an up-to-date overview on cell-SELEX technology, discussing the most recent advances with a particular focus on cancer cell targeting. Examples of the different protocol applications and post-SELEX strategies will be briefly outlined. View Full-Text
Keywords: aptamer; cell- Systematic Evolution of Ligands by Exponential enrichment; cell targeting aptamer; cell- Systematic Evolution of Ligands by Exponential enrichment; cell targeting
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Catuogno, S.; Esposito, C.L. Aptamer Cell-Based Selection: Overview and Advances. Biomedicines 2017, 5, 49.

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