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Chemosensors 2017, 5(4), 33; doi:10.3390/chemosensors5040033

Challenges in Developing a Biochip for Intact Histamine Using Commercial Antibodies

AIT Austrian Institute of Technology, Center for Health & Bioresources, Konrad Lorenz Str. 24, 3430 Tulln, Austria
Author to whom correspondence should be addressed.
Received: 16 October 2017 / Revised: 20 November 2017 / Accepted: 1 December 2017 / Published: 5 December 2017
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This study describes the development and the challenges in the development of an on-chip immunoassay for histamine using commercially available antibodies. Histamine can be used as an indicator of food freshness and quality, but it is also a relevant marker in clinical diagnostics. Due to its low molecular weight, simple structure and thus low immunogenicity production of high specificity and affinity antibodies is difficult. From six commercial anti-histamine antibodies tested, only two bound the histamine free in the solution. A fluorescent on-chip immunoassay for histamine was established with a dynamic range of 8–111 µg/mL using polyclonal anti-histamine antibody H7403 from Sigma (Mendota Heights, MN, USA). The anti-histamine antibodies described and used in published literature are thoroughly reviewed and the quality of commercial antibodies and their traceability and quality issues are highlighted and extensively discussed. View Full-Text
Keywords: histamine; immunoassay; biochip; antibody quality; traceability; polyclonal histamine; immunoassay; biochip; antibody quality; traceability; polyclonal

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Mattsson, L.; Doppler, S.; Preininger, C. Challenges in Developing a Biochip for Intact Histamine Using Commercial Antibodies. Chemosensors 2017, 5, 33.

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