Challenges in Developing a Biochip for Intact Histamine Using Commercial Antibodies
AbstractThis study describes the development and the challenges in the development of an on-chip immunoassay for histamine using commercially available antibodies. Histamine can be used as an indicator of food freshness and quality, but it is also a relevant marker in clinical diagnostics. Due to its low molecular weight, simple structure and thus low immunogenicity production of high specificity and affinity antibodies is difficult. From six commercial anti-histamine antibodies tested, only two bound the histamine free in the solution. A fluorescent on-chip immunoassay for histamine was established with a dynamic range of 8–111 µg/mL using polyclonal anti-histamine antibody H7403 from Sigma (Mendota Heights, MN, USA). The anti-histamine antibodies described and used in published literature are thoroughly reviewed and the quality of commercial antibodies and their traceability and quality issues are highlighted and extensively discussed. View Full-Text
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Mattsson, L.; Doppler, S.; Preininger, C. Challenges in Developing a Biochip for Intact Histamine Using Commercial Antibodies. Chemosensors 2017, 5, 33.
Mattsson L, Doppler S, Preininger C. Challenges in Developing a Biochip for Intact Histamine Using Commercial Antibodies. Chemosensors. 2017; 5(4):33.Chicago/Turabian Style
Mattsson, Leena; Doppler, Sara; Preininger, Claudia. 2017. "Challenges in Developing a Biochip for Intact Histamine Using Commercial Antibodies." Chemosensors 5, no. 4: 33.