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Chemosensors 2017, 5(2), 12; doi:10.3390/chemosensors5020012

A Chemically-Bound Glutathione Sensor Bioinspired by the Defense of Organisms against Heavy Metal Contamination: Optimization of the Immobilization Conditions

Departament d’Enginyeria Química i Química Analítica, Facultat de Química, Universitat de Barcelona, Martí i Franquès 1-11, E-08028 Barcelona, Spain
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Author to whom correspondence should be addressed.
Academic Editor: Manel Del Valle
Received: 23 February 2017 / Revised: 23 March 2017 / Accepted: 25 March 2017 / Published: 2 April 2017
(This article belongs to the Special Issue Bioinspired Chemical Sensing)
View Full-Text   |   Download PDF [1682 KB, uploaded 14 April 2017]   |  

Abstract

The influence of the experimental conditions (glutathione concentration and incubation time and temperature) concerning the covalent immobilization of glutathione via carbodiimide coupling on the behavior of a glutathione modified screen-printed carbon electrode obtained by electrografting is evaluated. The optimized parameters fasten the modification process and improve the performance of the sensor as compared to the usual procedure. This suggests the convenience of a tailored preparation of metal sensors based on metal-binding biomolecules such as glutathione. View Full-Text
Keywords: heavy metal sensors; voltammetry; glutathione; electrografting; screen-printed electrodes heavy metal sensors; voltammetry; glutathione; electrografting; screen-printed electrodes
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Pérez-Ràfols, C.; Serrano, N.; Díaz-Cruz, J.M.; Ariño, C.; Esteban, M. A Chemically-Bound Glutathione Sensor Bioinspired by the Defense of Organisms against Heavy Metal Contamination: Optimization of the Immobilization Conditions. Chemosensors 2017, 5, 12.

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