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Chemosensors 2016, 4(4), 21; doi:10.3390/chemosensors4040021

Molecularly Imprinted Quartz Crystal Microbalance Sensor (QCM) for Bilirubin Detection

1
Department of Chemistry, Hacettepe University, 06800 Ankara, Turkey
2
Department of Chemical Technology, Abant İzzet Baysal University, 14900 Bolu, Turkey
*
Author to whom correspondence should be addressed.
Academic Editor: Peter Lieberzeit
Received: 22 August 2016 / Revised: 24 October 2016 / Accepted: 31 October 2016 / Published: 18 November 2016
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Abstract

This study aims the preparation of a QCM sensor for the detection of bilirubin in human plasma. Bilirubin-imprinted poly-(2-hydroxyethyl methacrylate-N-methacryloyl-l-tryptophan methyl ester) (PHEMATrp) nanofilm (MIP) on the gold surface of a QCM chip was synthesized by the molecular imprinting technique. Meanwhile, the non-imprinted PHEMATrp (NIP) nanofilm was synthesized by the same experimental technique to examine the imprinting effect. Characterization of MIP and NIP nanofilms on the QCM chip surface was achieved by atomic force microscopy (AFM), ellipsometry, Fourier transform infrared spectrophotometry-attenuated total reflectance (FTIR-ATR) and contact angle measurements (CA). The observations indicated that the nanofilm was almost in a monolayer. Thereinafter, the imprinted and the non-imprinted QCM chips were connected to the QCM system to investigate kinetic and affinity properties. In order to examine the selectivity of the MIP-PHEMATrp nanofilm, competitive adsorption of bilirubin with cholesterol and estradiol was performed. Limit of detection (LOD) and limit of quantitation (LOQ) values were calculated as 0.45 μg/mL and 0.9 μg/mL, respectively. View Full-Text
Keywords: molecular imprinting technique; bilirubin; QCM; PHEMA; nanosensor molecular imprinting technique; bilirubin; QCM; PHEMA; nanosensor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Çiçek, Ç.; Yılmaz, F.; Özgür, E.; Yavuz, H.; Denizli, A. Molecularly Imprinted Quartz Crystal Microbalance Sensor (QCM) for Bilirubin Detection. Chemosensors 2016, 4, 21.

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