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Chemosensors 2015, 3(3), 211-223; doi:10.3390/chemosensors3030211

Steady-State Fluorescence and Lifetime Emission Study of pH-Sensitive Probes Based on i-motif Forming Oligonucleotides Single and Double Labeled with Pyrene

Adam Mickiewicz University, Faculty of Chemistry, Umultowska 89b, 61-614 Poznan, Poland
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Academic Editor: Igor Medintz
Received: 31 July 2015 / Accepted: 16 September 2015 / Published: 23 September 2015
(This article belongs to the Special Issue Feature Paper)
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Abstract

Cytosine-rich nucleic acids undergo pH-stimulated structural transitions leading to formation of an i-motif architecture at an acidic pH. Thus, i-motifs are good foundation for designing simple pH-sensitive fluorescent probes. We report here steady-state and time-resolved fluorescence studies of pyrene-labeled probes based on RET sequence: C4GC4GC4GC4TA (RET21), AC4GC4GC4GC4TA (RET21A) and C4GC4GC4GC4T (RET20). Comparative studies with single- and double-labeled i-motif probes were carried out. For each probe, we have measured fluorescence spectra and decays for emission wavelength of 390 nm over a wide range of pH (from 4.0 to 8.0). Effect of the oligonucleotide sequence and the number of pyrene labels on the spectral characteristics of probes were discussed. View Full-Text
Keywords: fluorescence; lifetime; pyrene; excimer; oligonucleotide probe; i-motif fluorescence; lifetime; pyrene; excimer; oligonucleotide probe; i-motif
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Dembska, A.; Rzepecka, P.; Juskowiak, B. Steady-State Fluorescence and Lifetime Emission Study of pH-Sensitive Probes Based on i-motif Forming Oligonucleotides Single and Double Labeled with Pyrene. Chemosensors 2015, 3, 211-223.

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