Previous Issue

Table of Contents

Proteomes, Volume 5, Issue 2 (June 2017)

  • Issues are regarded as officially published after their release is announced to the table of contents alert mailing list.
  • You may sign up for e-mail alerts to receive table of contents of newly released issues.
  • PDF is the official format for papers published in both, html and pdf forms. To view the papers in pdf format, click on the "PDF Full-text" link, and use the free Adobe Readerexternal link to open them.
View options order results:
result details:
Displaying articles 1-2
Export citation of selected articles as:

Editorial

Jump to: Review

Open AccessEditorial Clinical Proteomics: From Biological Sample to Clinical Exploitation
Proteomes 2017, 5(2), 10; doi:10.3390/proteomes5020010
Received: 5 April 2017 / Revised: 5 April 2017 / Accepted: 5 April 2017 / Published: 6 April 2017
PDF Full-text (139 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue Clinical Proteomics)

Review

Jump to: Editorial

Open AccessReview A Comprehensive Guide for Performing Sample Preparation and Top-Down Protein Analysis
Proteomes 2017, 5(2), 11; doi:10.3390/proteomes5020011
Received: 26 December 2016 / Revised: 4 April 2017 / Accepted: 4 April 2017 / Published: 7 April 2017
PDF Full-text (771 KB) | HTML Full-text | XML Full-text
Abstract
Methodologies for the global analysis of proteins in a sample, or proteome analysis, have been available since 1975 when Patrick O′Farrell published the first paper describing two-dimensional gel electrophoresis (2D-PAGE). This technique allowed the resolution of single protein isoforms, or proteoforms, into single
[...] Read more.
Methodologies for the global analysis of proteins in a sample, or proteome analysis, have been available since 1975 when Patrick O′Farrell published the first paper describing two-dimensional gel electrophoresis (2D-PAGE). This technique allowed the resolution of single protein isoforms, or proteoforms, into single ‘spots’ in a polyacrylamide gel, allowing the quantitation of changes in a proteoform′s abundance to ascertain changes in an organism′s phenotype when conditions change. In pursuit of the comprehensive profiling of the proteome, significant advances in technology have made the identification and quantitation of intact proteoforms from complex mixtures of proteins more routine, allowing analysis of the proteome from the ‘Top-Down’. However, the number of proteoforms detected by Top-Down methodologies such as 2D-PAGE or mass spectrometry has not significantly increased since O’Farrell’s paper when compared to Bottom-Up, peptide-centric techniques. This article explores and explains the numerous methodologies and technologies available to analyse the proteome from the Top-Down with a strong emphasis on the necessity to analyse intact proteoforms as a better indicator of changes in biology and phenotype. We arrive at the conclusion that the complete and comprehensive profiling of an organism′s proteome is still, at present, beyond our reach but the continuing evolution of protein fractionation techniques and mass spectrometry brings comprehensive Top-Down proteome profiling closer. Full article
Figures

Figure 1

Journal Contact

MDPI AG
Proteomes Editorial Office
St. Alban-Anlage 66, 4052 Basel, Switzerland
E-Mail: 
Tel. +41 61 683 77 34
Fax: +41 61 302 89 18
Editorial Board
Contact Details Submit to Proteomes Edit a special issue Review for Proteomes
loading...
Back to Top