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Proteomes 2017, 5(1), 1; doi:10.3390/proteomes5010001

Targeted Enlargement of Aptamer Functionalized Gold Nanoparticles for Quantitative Protein Analysis

Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2G3, Canada
Current Address: Department of Chemistry, Brock University, St. Catharines, ON L2S 3A1, Canada.
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Academic Editors: Jens R. Coorssen, Alfred L. Yergey and Jacek R. Wisniewski
Received: 1 November 2016 / Revised: 12 December 2016 / Accepted: 18 December 2016 / Published: 22 December 2016
View Full-Text   |   Download PDF [2066 KB, uploaded 22 December 2016]   |  

Abstract

The ability to selectively amplify the detection signals for targets over interferences is crucial when analyzing proteins in a complicated sample matrix. Here, we describe a targeted enlargement strategy that can amplify the light-scattering signal from aptamer-functionalized gold nanoparticles (Apt-AuNP) with high specificity for quantitative protein analysis. This strategy is achieved by labeling target proteins with competitively protected Apt-AuNP probes and enlarging the probes with gold enhancement. This competitive protection strategy could effectively eliminate nonspecific protein adsorptions from a sample matrix, leading to a highly specific labeling of the target protein. As a result, the subsequent amplification of the light-scattering signal by gold enhancement only occurs in the presence of the target protein. This strategy was successfully demonstrated by analyzing human α-thrombin in human serum samples in a Western blot format. View Full-Text
Keywords: aptamer; protein quantification; Western blot; gold enlargement aptamer; protein quantification; Western blot; gold enlargement
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Li, F.; Li, J.; Tang, Y.; Wang, C.; Li, X.-F.; Le, X.C. Targeted Enlargement of Aptamer Functionalized Gold Nanoparticles for Quantitative Protein Analysis. Proteomes 2017, 5, 1.

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