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Proteomes 2016, 4(3), 23; doi:10.3390/proteomes4030023

Comparative “Golgi” Proteome Study of Lolium multiflorum and Populus trichocarpa

1
Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia
2
Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 106 91 Stockholm, Sweden
3
Australian Research Council Centre of Excellence in Plant Cell Walls, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, SA 5064, Australia
*
Author to whom correspondence should be addressed.
Academic Editors: Nicolas L. Taylor and Jacek R. Wisniewski
Received: 23 May 2016 / Revised: 8 July 2016 / Accepted: 8 July 2016 / Published: 20 July 2016
(This article belongs to the Special Issue Sub-cellular Proteomics)
View Full-Text   |   Download PDF [2974 KB, uploaded 27 July 2016]   |  

Abstract

The Golgi apparatus (GA) is a crucial organelle in the biosynthesis of non-cellulosic polysaccharides, glycoproteins and proteoglycans that are primarily destined for secretion to the cell surface (plasma membrane, cell wall and apoplast). Only a small proportion of the proteins involved in these processes have been identified in plants, with the majority of their functions still unknown. The availability of a GA proteome would greatly assist plant biochemists, cell and molecular biologists in determining the precise function of the cell wall-related proteins. There has been some progress towards defining the GA proteome in the model plant system Arabidopsis thaliana, yet in commercially important species, such as either the cereals or woody species there has been relatively less progress. In this study, we applied discontinuous sucrose gradient centrifugation to partially enrich GA from suspension cell cultures (SCCs) and combined this with stable isotope labelling (iTRAQ) to determine protein sub-cellular locations. Results from a representative grass species, Italian ryegrass (Lolium multiflorum) and a dicot species, black cottonwood (Populus trichocarpa) are compared. The results confirm that membrane fractionation approaches that provide effective GA-enriched fractions for proteomic analyses in Arabidopsis are much less effective in the species examined here and highlight the complexity of the GA, both within and between species. View Full-Text
Keywords: Golgi apparatus; sub-cellular fractionation; subcellular proteomics; quantitative proteomics Golgi apparatus; sub-cellular fractionation; subcellular proteomics; quantitative proteomics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Ford, K.L.; Chin, T.; Srivastava, V.; Zeng, W.; Doblin, M.S.; Bulone, V.; Bacic, A. Comparative “Golgi” Proteome Study of Lolium multiflorum and Populus trichocarpa. Proteomes 2016, 4, 23.

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