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Proteomes 2016, 4(2), 15; doi:10.3390/proteomes4020015

Age- and Activity-Related Differences in the Abundance of Myosin Essential and Regulatory Light Chains in Human Muscle

Division of Sport and Exercise Science, Abertay University, 40 Bell Street, Kydd Building, Dundee DD1 1HG, UK
Faculty of Sport Science and Coaching, Universiti Pendidikan Sultan Idris, Tanjung Malim, Perak 35900, Malaysia
Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Jacek R. Wisniewsk
Received: 27 February 2016 / Revised: 30 March 2016 / Accepted: 1 April 2016 / Published: 8 April 2016
(This article belongs to the Special Issue Striated Muscle Proteomics)
View Full-Text   |   Download PDF [1977 KB, uploaded 8 April 2016]   |  


Traditional methods for phenotyping skeletal muscle (e.g., immunohistochemistry) are labor-intensive and ill-suited to multixplex analysis, i.e., assays must be performed in a series. Addressing these concerns represents a largely unmet research need but more comprehensive parallel analysis of myofibrillar proteins could advance knowledge regarding age- and activity-dependent changes in human muscle. We report a label-free, semi-automated and time efficient LC-MS proteomic workflow for phenotyping the myofibrillar proteome. Application of this workflow in old and young as well as trained and untrained human skeletal muscle yielded several novel observations that were subsequently verified by multiple reaction monitoring (MRM). We report novel data demonstrating that human ageing is associated with lesser myosin light chain 1 content and greater myosin light chain 3 content, consistent with an age-related reduction in type II muscle fibers. We also disambiguate conflicting data regarding myosin regulatory light chain, revealing that age-related changes in this protein more closely reflect physical activity status than ageing per se. This finding reinforces the need to control for physical activity levels when investigating the natural process of ageing. Taken together, our data confirm and extend knowledge regarding age- and activity-related phenotypes. In addition, the MRM transitions described here provide a methodological platform that can be fine-tuned to suite multiple research needs and thus advance myofibrillar phenotyping. View Full-Text
Keywords: ageing; human skeletal muscle; myosin heavy chain; myosin light chain; selective reaction monitoring ageing; human skeletal muscle; myosin heavy chain; myosin light chain; selective reaction monitoring

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Cobley, J.N.; Ab. Malik, Z.; Morton, J.P.; Close, G.L.; Edwards, B.J.; Burniston, J.G. Age- and Activity-Related Differences in the Abundance of Myosin Essential and Regulatory Light Chains in Human Muscle. Proteomes 2016, 4, 15.

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