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Proteomes 2015, 3(4), 347-368; doi:10.3390/proteomes3040347

The Cytosolic Oligosaccharide-Degrading Proteome of Butyrivibrio Proteoclasticus

Rumen Microbiology, Animal Science Group, AgResearch Limited, Grasslands Research Centre, Palmerston North 4442, New Zealand
Centre for Biodiscovery and School of Biological Sciences, Victoria University of Wellington, Wellington 6140, New Zealand
AgResearch Limited/Victoria University of Wellington Proteomics Laboratory, Victoria University of Wellington, Wellington 6140, New Zealand
Author to whom correspondence should be addressed.
Academic Editors: Michael Hecker and Katharina Riedel
Received: 31 August 2015 / Revised: 15 October 2015 / Accepted: 19 October 2015 / Published: 27 October 2015
(This article belongs to the Special Issue Microbial Proteomics)
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The growth and productivity of ruminants depends on a complex microbial community found in their fore-stomach (rumen), which is able to breakdown plant polysaccharides and ferment the released sugars. Butyrivibrio proteoclasticus B316T is a Gram-positive polysaccharide-degrading, butyrate-producing bacterium that is present at high numbers in the rumen of animals consuming pasture or grass silage based diets. B316T is one of a small number of rumen fibrolytic microbes capable of efficiently degrading and utilizing xylan, as well as being capable of utilizing arabinose, xylose, pectin and starch. We have therefore carried out a proteomic analysis of B316T to identify intracellular enzymes that are implicated in the metabolism of internalized xylan. Three hundred and ninety four proteins were identified including enzymes that have potential to metabolize assimilated products of extracellular xylan digestion. Identified enzymes included arabinosidases, esterases, an endoxylanase, and β-xylosidase. The presence of intracellular debranching enzymes indicated that some hemicellulosic side-chains may not be removed until oligosaccharides liberated by extracellular digestion have been assimilated by the cells. The results support a model of extracellular digestion of hemicellulose to oligosaccharides that are then transported to the cytoplasm for further digestion by intracellular enzymes. View Full-Text
Keywords: butyrivibrio proteoclasticus; carbohydrate active enzymes; hemicellulose; oligosaccharidases; proteomics; rumen; xylan butyrivibrio proteoclasticus; carbohydrate active enzymes; hemicellulose; oligosaccharidases; proteomics; rumen; xylan

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Dunne, J.C.; Kelly, W.J.; Leahy, S.C.; Li, D.; Bond, J.J.; Peng, L.; Attwood, G.T.; Jordan, T.W. The Cytosolic Oligosaccharide-Degrading Proteome of Butyrivibrio Proteoclasticus. Proteomes 2015, 3, 347-368.

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