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Proteomes 2015, 3(3), 236-248; doi:10.3390/proteomes3030236

Proteome Characterization of Leaves in Common Bean

1
Proteomics and Metabolomics Facility, Colorado State University, Fort Collins, CO 80523, USA
2
Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523, USA
3
Department of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, CO 80523, USA
4
Department of Biochemistry, Colorado State University, Fort Collins, CO 80523, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Jacek R. Wisniewski
Received: 17 June 2015 / Revised: 30 July 2015 / Accepted: 7 August 2015 / Published: 18 August 2015
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Abstract

Dry edible bean (Phaseolus vulgaris L.) is a globally relevant food crop. The bean genome was recently sequenced and annotated allowing for proteomics investigations aimed at characterization of leaf phenotypes important to agriculture. The objective of this study was to utilize a shotgun proteomics approach to characterize the leaf proteome and to identify protein abundance differences between two bean lines with known variation in their physiological resistance to biotic stresses. Overall, 640 proteins were confidently identified. Among these are proteins known to be involved in a variety of molecular functions including oxidoreductase activity, binding peroxidase activity, and hydrolase activity. Twenty nine proteins were found to significantly vary in abundance (p-value < 0.05) between the two bean lines, including proteins associated with biotic stress. To our knowledge, this work represents the first large scale shotgun proteomic analysis of beans and our results lay the groundwork for future studies designed to investigate the molecular mechanisms involved in pathogen resistance. View Full-Text
Keywords: shotgun proteomics; LC-MS; Phaseolus vulgaris; plant defense; pathogen resistance; beans; leaves shotgun proteomics; LC-MS; Phaseolus vulgaris; plant defense; pathogen resistance; beans; leaves
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Robison, F.M.; Heuberger, A.L.; Brick, M.A.; Prenni, J.E. Proteome Characterization of Leaves in Common Bean. Proteomes 2015, 3, 236-248.

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