Next Article in Journal
Activation of SsoPK4, an Archaeal eIF2α Kinase Homolog, by Oxidized CoA
Previous Article in Journal
S-Nitrosylation in Organs of Mice Exposed to Low or High Doses of γ-Rays: The Modulating Effect of Iodine Contrast Agent at a Low Radiation Dose
Article Menu

Export Article

Open AccessArticle
Proteomes 2015, 3(2), 74-88; doi:10.3390/proteomes3020074

Regional Specializations of the PAZ Proteomes Derived from Mouse Hippocampus, Olfactory Bulb and Cerebellum

1
Department Molecular and Cellular Neurobiology, Institute for Cell Biology and Neuroscience, Goethe University Frankfurt am Main, Max-von-Laue-Straße 13, 60438 Frankfurt am Main, Germany
2
Institute of Pharmaceutical Chemistry, Goethe University Frankfurt am Main, Max-von-Laue-Straße 9; 60438 Frankfurt am Main, Germany
3
SunChrom Wissenschaftliche Geräte GmbH, Industriestraße 27, 61381 Friedrichsdorf, Germany
4
Paul-Ehrlich-Institute, German Federal Institute for Drugs and Medicinal Products, Paul-Ehrlich-Straße 51-59, 63225 Langen, Germany
These authors contributed equally to this work.
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 26 January 2015 / Accepted: 5 May 2015 / Published: 13 May 2015
View Full-Text   |   Download PDF [1687 KB, uploaded 13 May 2015]   |  

Abstract

Neurotransmitter release as well as structural and functional dynamics at the presynaptic active zone (PAZ) comprising synaptic vesicles attached to the presynaptic plasma membrane are mediated and controlled by its proteinaceous components. Here we describe a novel experimental design to immunopurify the native PAZ-complex from individual mouse brain regions such as olfactory bulb, hippocampus, and cerebellum with high purity that is essential for comparing their proteome composition. Interestingly, quantitative immunodetection demonstrates significant differences in the abundance of prominent calcium-dependent PAZ constituents. Furthermore, we characterized the proteomes of the immunoisolated PAZ derived from the three brain regions by mass spectrometry. The proteomes of the release sites from the respective regions exhibited remarkable differences in the abundance of a large variety of PAZ constituents involved in various functional aspects of the release sites such as calcium homeostasis, synaptic plasticity and neurogenesis. On the one hand, our data support an identical core architecture of the PAZ for all brain regions and, on the other hand, demonstrate that the proteinaceous composition of their presynaptic active zones vary, suggesting that changes in abundance of individual proteins strengthen the ability of the release sites to adapt to specific functional requirements. View Full-Text
Keywords: cerebellum; hippocampus; olfactory bulb; proteome; presynaptic active zone cerebellum; hippocampus; olfactory bulb; proteome; presynaptic active zone
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Weingarten, J.; Laßek, M.; Mueller, B.F.; Rohmer, M.; Baeumlisberger, D.; Beckert, B.; Ade, J.; Gogesch, P.; Acker-Palmer, A.; Karas, M.; Volknandt, W. Regional Specializations of the PAZ Proteomes Derived from Mouse Hippocampus, Olfactory Bulb and Cerebellum. Proteomes 2015, 3, 74-88.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Proteomes EISSN 2227-7382 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top