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Proteomes 2014, 2(2), 258-271; doi:10.3390/proteomes2020258
Article

Protein Profiling Reveals Novel Proteins in Pollen and Pistil of W22 (ga1; Ga1) in Maize

1,†
, 1,†
, 1,†,‡
, 2
, 1
, 3
, 1
, 4
 and 1,*
1 Department of Crop Science, Chungbuk National University, Cheong-ju 361-763, Korea 2 Division of Mass Spectrometry Research, Korea Basic Science Institute, Chungbuk 863-883, Korea 3 Lab of Molecular Breeding, Arid land Research Center, Tottori University, Tottori 680-8550, Japan 4 USDA-ARS Plant Science Research Unit, Department of Crop Science, Box 7620, North Carolina State University, Raleigh, NC 27695, USA These authors contributed equally to this work. Current address: National Institute of Crop Science, NARO, Tsukuba 305-8517, Japan.
* Author to whom correspondence should be addressed.
Received: 8 February 2014 / Revised: 8 April 2014 / Accepted: 21 April 2014 / Published: 5 May 2014
(This article belongs to the Special Issue Plant Proteomics)
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Abstract

Gametophytic factors mediate pollen-pistil interactions in maize (Zea mays L.) and play active roles in limiting gene flow among maize populations and between maize and teosinte. This study was carried out to identify proteins and investigate the mechanism of gametophytic factors using protein analysis. W22 (ga1); which did not carry a gametophytic factor and W22 (Ga1), a near iso-genic line, were used for the proteome investigation. SDS-PAGE was executed to investigate proteins in the pollen and pistil of W22 (ga1) and W22 (Ga1). A total of 44 differentially expressed proteins were identified in the pollen and pistil on SDS-PAGE using LTQ-FTICR MS. Among the 44 proteins, a total of 24 proteins were identified in the pollen of W22 (ga1) and W22 (Ga1) whereas 20 differentially expressed proteins were identified from the pistil of W22 (ga1) and W22 (Ga1). However, in pollen, 2 proteins were identified only in the W22 (ga1) and 12 proteins only in the W22 (Ga1) whereas 10 proteins were confirmed from the both of W22 (ga1) and W22 (Ga1). In contrary, 10 proteins were appeared only in the pistil of W22 (ga1) and 7 proteins from W22 (Ga1) while 3 proteins confirmed in the both of W22 (ga1) and W22 (Ga1). Moreover, the identified proteins were generally involved in hydrolase activity, nucleic acid binding and nucleotide binding. These results help to reveal the mechanism of gametophytic factors and provide a valuable clue for the pollen and pistil research in maize.
Keywords: maize; pollen; pistil; gametophytic factors; proteomics maize; pollen; pistil; gametophytic factors; proteomics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Yu, J.; Roy, S.K.; Kamal, A.H.M.; Cho, K.; Kwon, S.-J.; Cho, S.-W.; So, Y.-S.; Holland, J.B.; Woo, S.H. Protein Profiling Reveals Novel Proteins in Pollen and Pistil of W22 (ga1; Ga1) in Maize. Proteomes 2014, 2, 258-271.

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